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Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion

Overview of attention for article published in AMB Express, December 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (54th percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

Mentioned by

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2 tweeters

Citations

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10 Dimensions

Readers on

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63 Mendeley
Title
Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Published in
AMB Express, December 2015
DOI 10.1186/s13568-015-0170-z
Pubmed ID
Authors

Juliana de Amorim Araújo, Túlio César Ferreira, Marciano Régis Rubini, Ana Gilhema Gomez Duran, Janice Lisboa De Marco, Lidia Maria Pepe de Moraes, Fernando Araripe Gonçalves Torres

Abstract

The term cellulase refers to any component of the enzymatic complex produced by some fungi, bacteria and protozoans which act serially or synergistically to catalyze the cleavage of cellulosic materials. Cellulases have been widely used in many industrial applications ranging from food industry to the production of second generation ethanol. In an effort to develop new strategies to minimize the costs of enzyme production we describe the development of a Pichia pastoris strain able to coproduce two different cellulases. For that purpose the eglII (endoglucanase II) and cbhII (cellobiohydrolase II) genes from Trichoderma reesei were fused in-frame separated by the self-processing 2A peptide sequence from the foot-and-mouth disease virus. The protein fusion construct was placed under the control of the strong inducible AOX1 promoter. Analysis of culture supernatants from methanol-induced yeast transformants showed that the protein fusion was effectively processed. Enzymatic assay showed that the processed enzymes were fully functional with the same catalytic properties of the individual enzymes produced separately. Furthermore, when combined both enzymes acted synergistically on filter paper to produce cellobiose as the main end-product. Based on these results we propose that P. pastoris should be considered as an alternative platform for the production of cellulases at competitive costs.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Austria 1 2%
Unknown 61 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 13 21%
Student > Bachelor 10 16%
Student > Ph. D. Student 9 14%
Researcher 9 14%
Professor > Associate Professor 3 5%
Other 11 17%
Unknown 8 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 44%
Biochemistry, Genetics and Molecular Biology 14 22%
Social Sciences 2 3%
Chemistry 2 3%
Immunology and Microbiology 1 2%
Other 5 8%
Unknown 11 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 December 2015.
All research outputs
#2,935,111
of 6,865,651 outputs
Outputs from AMB Express
#73
of 342 outputs
Outputs of similar age
#123,962
of 295,814 outputs
Outputs of similar age from AMB Express
#8
of 29 outputs
Altmetric has tracked 6,865,651 research outputs across all sources so far. This one has received more attention than most of these and is in the 54th percentile.
So far Altmetric has tracked 342 research outputs from this source. They receive a mean Attention Score of 2.0. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 295,814 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.