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An integrated chip-mass spectrometry and epifluorescence approach for online monitoring of bioactive metabolites from incubated Actinobacteria in picoliter droplets

Overview of attention for article published in Analytical & Bioanalytical Chemistry, September 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (57th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

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7 tweeters

Citations

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8 Dimensions

Readers on

mendeley
19 Mendeley
Title
An integrated chip-mass spectrometry and epifluorescence approach for online monitoring of bioactive metabolites from incubated Actinobacteria in picoliter droplets
Published in
Analytical & Bioanalytical Chemistry, September 2018
DOI 10.1007/s00216-018-1383-1
Pubmed ID
Authors

Konstantin Wink, Lisa Mahler, Julia R. Beulig, Sebastian K. Piendl, Martin Roth, Detlev Belder

Abstract

We present a lab-on-a-chip approach for the analysis of secondary metabolites produced in microfluidic droplets by simultaneous epifluorescence microscopy and electrospray ionization mass spectrometry (ESI-MS). The approach includes encapsulation and long-term off-chip incubation of microbes in surfactant-stabilized droplets followed by a transfer of droplets into a microfluidic chip for subsequent analysis. Before the reinjected droplets are spaced and electrosprayed from an integrated emitter into a mass spectrometer, the presence of fluorescent marker molecules is monitored nearly simultaneously with a custom-made portable epifluorescence microscope. This combined fluorescence and MS-detection setup allows the analysis of metabolites and fluorescent labels in a complex biological matrix at a single droplet level. Using hyphae of Streptomyces griseus, encapsulated in microfluidic droplets of ~ 200 picoliter as a model system, we show the detection of in situ produced streptomycin by ESI-MS and the feasibility of detecting fluorophores inside droplets shortly before they are electrosprayed. The presented method expands the analytical toolbox for the discovery of bioactive metabolites such as novel antibiotics, produced by microorganisms.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 47%
Student > Master 3 16%
Researcher 2 11%
Student > Bachelor 2 11%
Student > Doctoral Student 1 5%
Other 0 0%
Unknown 2 11%
Readers by discipline Count As %
Chemistry 7 37%
Chemical Engineering 2 11%
Biochemistry, Genetics and Molecular Biology 2 11%
Engineering 2 11%
Medicine and Dentistry 1 5%
Other 2 11%
Unknown 3 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2018.
All research outputs
#7,105,684
of 13,869,998 outputs
Outputs from Analytical & Bioanalytical Chemistry
#1,842
of 5,226 outputs
Outputs of similar age
#112,948
of 271,495 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#20
of 226 outputs
Altmetric has tracked 13,869,998 research outputs across all sources so far. This one is in the 48th percentile – i.e., 48% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,226 research outputs from this source. They receive a mean Attention Score of 2.5. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,495 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 226 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.