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Expression and characterization of human proinsulin fused to thioredoxin in Escherichia coli

Overview of attention for article published in Applied Microbiology & Biotechnology, December 2011
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

Mentioned by

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1 tweeter
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1 patent

Citations

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11 Dimensions

Readers on

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12 Mendeley
Title
Expression and characterization of human proinsulin fused to thioredoxin in Escherichia coli
Published in
Applied Microbiology & Biotechnology, December 2011
DOI 10.1007/s00253-011-3721-5
Pubmed ID
Authors

Aldana Trabucchi, Luciano Lucas Guerra, Natalia Inés Faccinetti, Ruben Francisco Iacono, Edgardo Poskus, Silvina Noemí Valdez

Abstract

Native proinsulin (PI) belongs to the class of the difficult-to-express proteins in Escherichia coli. Problems mainly arise due to its high proteolytic decay and troubles to reproduce the native disulphide pattern. In the present study, human PI was produced in E. coli as a fusion thioredoxin protein (Trx-PI). Such chimeric protein was obtained from the intracellular soluble fraction, and it was purified in one step by affinity chromatography on immobilized phenylarsine oxide. Trx-PI was also recovered from inclusion bodies and purified by anion exchange chromatography. The product identity and integrity were verified by mass analysis (22,173.5 Da) and mapping with Staphylococcus aureus V8 protease. Native PI folding was evaluated by biochemical and also by immunochemical analysis using specific sera from PI antibody-positive diabetic patients that recognise conformational discontinue epitopes. Dose-response curves showed identity between standard PI and Trx-PI. Moreover, surface plasmon resonance technique verified the correct conformation of the recombinant protein. The biochemical and immunochemical assays demonstrated the integrity of the chimera and the epitopes involved in the interaction with antibodies. In conclusion, it was possible to obtain with high-yield purified human PI as a fusion protein in E. coli and useful for analytical purposes.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Switzerland 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Student > Bachelor 3 25%
Researcher 2 17%
Student > Doctoral Student 2 17%
Other 1 8%
Other 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 42%
Medicine and Dentistry 2 17%
Veterinary Science and Veterinary Medicine 1 8%
Nursing and Health Professions 1 8%
Immunology and Microbiology 1 8%
Other 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 June 2013.
All research outputs
#3,670,456
of 13,243,449 outputs
Outputs from Applied Microbiology & Biotechnology
#1,404
of 5,482 outputs
Outputs of similar age
#51,403
of 205,910 outputs
Outputs of similar age from Applied Microbiology & Biotechnology
#18
of 46 outputs
Altmetric has tracked 13,243,449 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 5,482 research outputs from this source. They receive a mean Attention Score of 3.6. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 205,910 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.