| Title |
Metabolic engineering of Saccharomyces cerevisiae for linalool production
|
|---|---|
| Published in |
Biotechnology Techniques, November 2015
|
| DOI | 10.1007/s10529-015-2000-4 |
| Pubmed ID | |
| Authors |
Pegah Amiri, Azar Shahpiri, Mohammad Ali Asadollahi, Fariborz Momenbeik, Siavash Partow |
| Abstract |
To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool. Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its native promoter with the repressible MET3 promoter in the presence of methionine resulted in accumulation of 78 µg linalool l(-1) in the culture medium. This was more than twice that produced by the control strain. The highest linalool titer was obtained by combined repression of ERG9 and overexpression of tHMG1. The yeast strain harboring both modifications produced 95 μg linalool l(-1). Although overexpression of tHMG1 and downregulation of ERG9 enhanced linalool titers threefold in the engineered yeast strain, alleviating linalool toxicity is necessary for further improvement of linalool biosynthesis in yeast. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 115 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | <1% |
| France | 1 | <1% |
| Unknown | 113 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 19% |
| Researcher | 19 | 17% |
| Student > Bachelor | 18 | 16% |
| Student > Master | 13 | 11% |
| Student > Postgraduate | 8 | 7% |
| Other | 11 | 10% |
| Unknown | 24 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 34 | 30% |
| Agricultural and Biological Sciences | 30 | 26% |
| Chemistry | 9 | 8% |
| Engineering | 6 | 5% |
| Immunology and Microbiology | 3 | 3% |
| Other | 5 | 4% |
| Unknown | 28 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2015.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Biotechnology Techniques
#2,492
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Outputs of similar age
#336,542
of 394,393 outputs
Outputs of similar age from Biotechnology Techniques
#23
of 33 outputs
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