Title |
Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patients
|
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Published in |
SpringerPlus, October 2015
|
DOI | 10.1186/s40064-015-1423-7 |
Pubmed ID | |
Authors |
Chieko Mishima, Naofumi Kagara, Saki Matsui, Tomonori Tanei, Yasuto Naoi, Masafumi Shimoda, Atsushi Shimomura, Kenzo Shimazu, Seung Jin Kim, Shinzaburo Noguchi |
Abstract |
The aim of the present study was to investigate the promoter methylation status of TRIM9 in breast cancer and to determine the presence of TRIM9-methylated circulating tumor DNA (ctDNA) in plasma. Bisulfite sequencing with a next generation sequencer showed TRIM9 promoter methylation in 92 % (11/12) of breast cancer cell lines (BCCs) and 68 % (13/19) of breast tumor tissues but not in any normal breast tissues (0/19). Methylation ratio of TRIM9 was significantly lower in basal type (9 %, n = 23) than luminal A (69 %, n = 29, P = 0.0003). Quantitative RT-PCR of BCCs disclosed an inverse correlation between TRIM9 mRNA expression and methylation ratio. TRIM9 methylated ctDNA in plasma was detected in 18 % (10/56) of metastatic breast cancer patients but not in any of 60 healthy controls. These results indicate that TRIM9 promoter hypermethylation, which suppresses TRIM9 mRNA expression, occurs in a significant proportion of breast tumors, and that TRIM9-methylated ctDNA thus may serve as a tumor marker for breast cancer. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 14% |
United Kingdom | 1 | 7% |
Unknown | 11 | 79% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 9 | 64% |
Practitioners (doctors, other healthcare professionals) | 3 | 21% |
Scientists | 2 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Ireland | 1 | 2% |
Unknown | 44 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 11 | 24% |
Student > Bachelor | 8 | 18% |
Student > Ph. D. Student | 6 | 13% |
Researcher | 5 | 11% |
Student > Doctoral Student | 4 | 9% |
Other | 5 | 11% |
Unknown | 6 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 17 | 38% |
Agricultural and Biological Sciences | 10 | 22% |
Medicine and Dentistry | 10 | 22% |
Neuroscience | 2 | 4% |
Unknown | 6 | 13% |