Title |
Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroids
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Published in |
Journal of Materials Science: Materials in Medicine, October 2015
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DOI | 10.1007/s10856-015-5591-3 |
Pubmed ID | |
Authors |
Hideyuki Suenaga, Katsuko S. Furukawa, Yukako Suzuki, Tsuyoshi Takato, Takashi Ushida |
Abstract |
Mesenchymal stem cell (MSC) condensation contributes to membrane ossification by enhancing their osteodifferentiation. We investigated bone regeneration in rats using the human bone marrow-derived MSC-spheroids prepared by rotation culture, without synthetic or exogenous biomaterials. Bilateral calvarial defects (8 mm) were created in nude male rats; the left-sided defects were implanted with MSC-spheroids, β-tricalcium phosphate (β-TCP) granules, or β-TCP granules + MSC-spheroids, while the right-sided defects served as internal controls. Micro-computed tomography and immunohistochemical staining for osteocalcin/osteopontin indicated formation of new, full-thickness bones at the implantation sites, but not at the control sites in the MSC-spheroid group. Raman spectroscopy revealed similarity in the spectral properties of the repaired bone and native calvarial bone. Mechanical performance of the bones in the MSC-implanted group was good (50 and 60 % those of native bones, respectively). All tests showed poor bone regeneration in the β-TCP and β-TCP + MSC-spheroid groups. Thus, significant bone regeneration was achieved with MSC-spheroid implantation into bone defects, justifying further investigation. |
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Country | Count | As % |
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Russia | 1 | 25% |
Australia | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
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Members of the public | 3 | 75% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
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Spain | 1 | <1% |
Unknown | 116 | 99% |
Demographic breakdown
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Student > Master | 23 | 20% |
Student > Ph. D. Student | 19 | 16% |
Student > Bachelor | 14 | 12% |
Researcher | 10 | 9% |
Student > Doctoral Student | 8 | 7% |
Other | 15 | 13% |
Unknown | 28 | 24% |
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Biochemistry, Genetics and Molecular Biology | 13 | 11% |
Agricultural and Biological Sciences | 13 | 11% |
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Materials Science | 6 | 5% |
Other | 14 | 12% |
Unknown | 36 | 31% |