Title |
DNA damage in blood lymphocytes in patients after 177Lu peptide receptor radionuclide therapy
|
---|---|
Published in |
European Journal of Nuclear Medicine and Molecular Imaging, June 2015
|
DOI | 10.1007/s00259-015-3083-9 |
Pubmed ID | |
Authors |
Uta Eberlein, Carina Nowak, Christina Bluemel, Andreas Konrad Buck, Rudolf Alexander Werner, Harry Scherthan, Michael Lassmann |
Abstract |
The aim of the study was to investigate DNA double strand break (DSB) formation and its correlation with the absorbed dose to the blood lymphocytes of patients undergoing their first peptide receptor radionuclide therapy (PRRT) with (177)Lu-labelled DOTATATE/DOTATOC. The study group comprised 16 patients receiving their first PRRT. At least six peripheral blood samples were obtained before, and between 0.5 h and 48 h after radionuclide administration. From the time-activity curves of the blood and the whole body, residence times for blood self-irradiation and whole-body irradiation were determined. Peripheral blood lymphocytes were isolated, fixed with ethanol and subjected to immunofluorescence staining for colocalizing γ-H2AX/53BP1 DSB-marking foci. The average number of DSB foci per cell per patient sample was determined as a function of the absorbed dose to the blood and compared with an in vitro calibration curve established in our laboratory with (131)I and (177)Lu. The average number of radiation-induced foci (RIF) per cell increased over the first 5 h after radionuclide administration and decreased thereafter. A linear fit from 0 to 5 h as a function of the absorbed dose to the blood agreed with our in vitro calibration curve. At later time-points the number of RIF decreased, indicating progression of DNA repair. Measurements of RIF and the absorbed dose to the blood after systemic administration of (177)Lu may be used to obtain data on the individual dose-response relationships in vivo. Individual patient data were characterized by a linear dose-dependent increase and an exponential decay function describing repair. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 26% |
Student > Ph. D. Student | 4 | 15% |
Student > Postgraduate | 2 | 7% |
Student > Master | 2 | 7% |
Student > Bachelor | 1 | 4% |
Other | 4 | 15% |
Unknown | 7 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 22% |
Biochemistry, Genetics and Molecular Biology | 3 | 11% |
Agricultural and Biological Sciences | 3 | 11% |
Physics and Astronomy | 3 | 11% |
Nursing and Health Professions | 1 | 4% |
Other | 4 | 15% |
Unknown | 7 | 26% |