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NMR resonance assignments for the SAM/SAH-binding riboswitch RNA bound to S-adenosylhomocysteine

Overview of attention for article published in Biomolecular NMR Assignments, July 2018
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Title
NMR resonance assignments for the SAM/SAH-binding riboswitch RNA bound to S-adenosylhomocysteine
Published in
Biomolecular NMR Assignments, July 2018
DOI 10.1007/s12104-018-9834-3
Pubmed ID
Authors

A. Katharina Weickhmann, Heiko Keller, Elke Duchardt-Ferner, Elisabeth Strebitzer, Michael A. Juen, Johannes Kremser, Jan Philip Wurm, Christoph Kreutz, Jens Wöhnert

Abstract

Riboswitches are structured RNA elements in the 5'-untranslated regions of bacterial mRNAs that are able to control the transcription or translation of these mRNAs in response to the specific binding of small molecules such as certain metabolites. Riboswitches that bind with high specificity to either S-adenosylmethionine (SAM) or S-adenosylhomocysteine (SAH) are widespread in bacteria. Based on differences in secondary structure and sequence these riboswitches can be grouped into a number of distinct classes. X-ray structures for riboswitch RNAs in complex with SAM or SAH established a structural basis for understanding ligand recognition and discrimination in many of these riboswitch classes. One class of riboswitches-the so-called SAM/SAH riboswitch class-binds SAM and SAH with similar affinity. However, this class of riboswitches is structurally not yet characterized and the structural basis for its unusual bispecificity is not established. In order to understand the ligand recognition mode that enables this riboswitch to bind both SAM and SAH with similar affinities, we are currently determining its structure in complex with SAH using NMR spectroscopy. Here, we present the NMR resonance assignment of the SAM/SAH binding riboswitch (env9b) in complex with SAH as a prerequisite for a solution NMR-based high-resolution structure determination.

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The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 3 43%
Researcher 1 14%
Student > Bachelor 1 14%
Student > Doctoral Student 1 14%
Student > Ph. D. Student 1 14%
Other 0 0%
Readers by discipline Count As %
Unspecified 3 43%
Chemistry 2 29%
Agricultural and Biological Sciences 1 14%
Biochemistry, Genetics and Molecular Biology 1 14%

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This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2018.
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#10,583,427
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Outputs from Biomolecular NMR Assignments
#66
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#200,564
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