| Title |
Genetic characterization of extraintestinal Escherichia coli isolates from chicken, cow and swine
|
|---|---|
| Published in |
AMB Express, July 2018
|
| DOI | 10.1186/s13568-018-0646-8 |
| Pubmed ID | |
| Authors |
Li Chen, Leyi Wang, Afrah Kamal Yassin, Jilei Zhang, Jiansen Gong, Kezong Qi, Roman R. Ganta, Yuanyuan Zhang, Yi Yang, Xiangan Han, Chengming Wang |
| Abstract |
Phenotypic determination of antimicrobial resistance in bacteria is very important for diagnosis and treatment, but sometimes this procedure needs further genetic evaluation. Whole-genome sequencing plays a critical role in deciphering and advancing our understanding of bacterial evolution, transmission, and surveillance of antimicrobial resistance. In this study, whole-genome sequencing was performed on nineteen clinically extraintestinal Escherichia coli isolates from chicken, cows and swine and showing different antimicrobial susceptibility. A total of 44 different genes conferring resistance to 11 classes of antimicrobials were detected in 15 of 19 E. coli isolates (78.9%), and 22 types of plasmids were detected in 15/19 (78.9%) isolates. In addition, whole-genome sequencing of these 19 isolates identified 111 potential virulence factors, and 53 of these VFDB-annotated genes were carried by all these 19 isolates. Twelve different virulence genes were identified while the most frequent ones were gad (glutamate decarboxylase), iss (increased serum survival) and lpfA (long polar fimbriae). All isolates harbored at least one of the virulence genes. The findings from comparative genomic analyses of the 19 diverse E. coli isolates in this study provided insights into molecular basis of the rising multi-drug resistance in E. coli. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 65 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 20% |
| Researcher | 13 | 20% |
| Student > Ph. D. Student | 11 | 17% |
| Student > Postgraduate | 4 | 6% |
| Student > Bachelor | 3 | 5% |
| Other | 7 | 11% |
| Unknown | 14 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 11 | 17% |
| Immunology and Microbiology | 10 | 15% |
| Biochemistry, Genetics and Molecular Biology | 6 | 9% |
| Medicine and Dentistry | 5 | 8% |
| Environmental Science | 4 | 6% |
| Other | 11 | 17% |
| Unknown | 18 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 July 2018.
All research outputs
#18,643,992
of 23,096,849 outputs
Outputs from AMB Express
#808
of 1,245 outputs
Outputs of similar age
#229,543
of 296,625 outputs
Outputs of similar age from AMB Express
#17
of 48 outputs
Altmetric has tracked 23,096,849 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,245 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 296,625 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.