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Evaluation of three extraction methods for molecular detection of Schistosoma mansoni infection in human urine and serum samples

Overview of attention for article published in Journal of Parasitic Diseases, November 2013
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  • Good Attention Score compared to outputs of the same age and source (74th percentile)

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2 tweeters

Citations

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4 Dimensions

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11 Mendeley
Title
Evaluation of three extraction methods for molecular detection of Schistosoma mansoni infection in human urine and serum samples
Published in
Journal of Parasitic Diseases, November 2013
DOI 10.1007/s12639-013-0385-3
Pubmed ID
Authors

Rania M. Sarhan, Hanan H. Kamel, Ghada A. Saad, Ossama A. Ahmed

Abstract

The diagnostic techniques based on polymerase chain reaction (PCR) for the detection of Schistosoma spp. DNA in stool, serum, plasma and urine has shown high sensitivity and specificity solving the problems for the low worm burdens and low transmission rates facing the routine microscopic diagnosis. Since PCR assays require efficient unbiased procedures of extraction and purification of nucleic acids. This study compared the efficiencies of simple, manual and feasible DNA extraction methods; a salting out and resin method, phenol/chloroform method to a commercial extraction kit through PCR analysis of human urine and serum samples spiked with known amounts of adult Schistosoma mansoni DNA confirmed by the application on real samples from patients. In artificially spiked urine gradient, the best mean diagnostic performance was that of salting out and resin then phenol/chloroform and last for the commercial kit. All three methods gave positive results in all tested urine samples which insures comparable high efficiency for DNA detection. In artificially spiked serum gradient, the highest mean diagnostic performance was obtained by the kit then salting out and resin and last by phenol chloroform. In patients' urine samples the phenol/chloroform method showed the highest mean diagnostic performance followed by the resin and then the kit. Using patients' serum samples the resin method showed equal mean diagnostic performance with the phenol/chloroform method which was higher compared to the kit. As regards sensitivity from urine samples the resin and phenol/chloroform showed equal results using artificial gradients and patients' samples. In serum samples the resin and phenol/chloroform showed equal results using artificial gradients while the resin showed better results in patients' samples. It is recommended to extract DNA from urine samples and to use the salting out and resin as a manual DNA extraction method from patients' samples for the molecular diagnosis of Schistosoma mansoni infection.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 27%
Researcher 2 18%
Student > Postgraduate 2 18%
Librarian 1 9%
Student > Ph. D. Student 1 9%
Other 2 18%
Readers by discipline Count As %
Unspecified 3 27%
Biochemistry, Genetics and Molecular Biology 2 18%
Medicine and Dentistry 2 18%
Agricultural and Biological Sciences 2 18%
Nursing and Health Professions 1 9%
Other 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2015.
All research outputs
#9,577,155
of 12,466,607 outputs
Outputs from Journal of Parasitic Diseases
#79
of 256 outputs
Outputs of similar age
#151,378
of 240,693 outputs
Outputs of similar age from Journal of Parasitic Diseases
#8
of 39 outputs
Altmetric has tracked 12,466,607 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 256 research outputs from this source. They receive a mean Attention Score of 1.1. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,693 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.