↓ Skip to main content

Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry

Overview of attention for article published in Analytical & Bioanalytical Chemistry, August 2015
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (77th percentile)

Mentioned by

twitter
4 tweeters

Citations

dimensions_citation
5 Dimensions

Readers on

mendeley
35 Mendeley
Title
Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry
Published in
Analytical & Bioanalytical Chemistry, August 2015
DOI 10.1007/s00216-015-8932-7
Pubmed ID
Authors

Saira Hameed, Koji Ikegami, Eiji Sugiyama, Shoko Matsushita, Yoshishige Kimura, Takahiro Hayasaka, Yuki Sugiura, Noritaka Masaki, Michihiko Waki, Isao Ohta, Md Amir Hossen, Mitsutoshi Setou

Abstract

A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Biomolecules in the m/z range from 700 to 900 were more effectively detected in the freeze-cracked than from simple frozen adult nematode bodies. Different distribution of biomolecules was observed in a nematode body when the matrix was applied with a sublimation deposition method. The whole-body IMS technique was applied on genetically deficient mutant C. elegans to combine whole-body lipidomics and genetics, by comparing the fatty acid compositions, especially of the phosphatidylcholine (PC) species, between the wild-type and fat-1 mutants, which lack the gene encoding an n-3 fatty acid desaturase. A significant reduction of PC(20:5/20:5) and PC(20:4/20:5) and a marked increase of PC(20:4/20:4), PC(20:3/20:4), and PC(20:3/20:3) were detected in the fat-1 mutants in positive ion mode. In addition, phospholipid compositions other than PCs were analyzed in negative ion mode. A loss of a possible phosphatidylinositol (PI) with 18:0/20:5 and a compensative accumulation of putative PI(18:0/20:4) were detected in the fat-1 mutants. In conclusion, the whole-body MALDI-IMS technique is useful for the profiling of multiple biomolecules in C. elegans in both intra- and inter-individual levels.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 26%
Researcher 8 23%
Student > Bachelor 5 14%
Other 4 11%
Unspecified 3 9%
Other 6 17%
Readers by discipline Count As %
Chemistry 9 26%
Agricultural and Biological Sciences 7 20%
Biochemistry, Genetics and Molecular Biology 6 17%
Unspecified 5 14%
Engineering 2 6%
Other 6 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2016.
All research outputs
#7,642,986
of 12,230,555 outputs
Outputs from Analytical & Bioanalytical Chemistry
#2,436
of 4,657 outputs
Outputs of similar age
#128,911
of 240,789 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#54
of 260 outputs
Altmetric has tracked 12,230,555 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,657 research outputs from this source. They receive a mean Attention Score of 2.4. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,789 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 260 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.