| Title |
Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, August 2015
|
| DOI | 10.1007/s00216-015-8932-7 |
| Pubmed ID | |
| Authors |
Saira Hameed, Koji Ikegami, Eiji Sugiyama, Shoko Matsushita, Yoshishige Kimura, Takahiro Hayasaka, Yuki Sugiura, Noritaka Masaki, Michihiko Waki, Isao Ohta, Md Amir Hossen, Mitsutoshi Setou |
| Abstract |
A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Biomolecules in the m/z range from 700 to 900 were more effectively detected in the freeze-cracked than from simple frozen adult nematode bodies. Different distribution of biomolecules was observed in a nematode body when the matrix was applied with a sublimation deposition method. The whole-body IMS technique was applied on genetically deficient mutant C. elegans to combine whole-body lipidomics and genetics, by comparing the fatty acid compositions, especially of the phosphatidylcholine (PC) species, between the wild-type and fat-1 mutants, which lack the gene encoding an n-3 fatty acid desaturase. A significant reduction of PC(20:5/20:5) and PC(20:4/20:5) and a marked increase of PC(20:4/20:4), PC(20:3/20:4), and PC(20:3/20:3) were detected in the fat-1 mutants in positive ion mode. In addition, phospholipid compositions other than PCs were analyzed in negative ion mode. A loss of a possible phosphatidylinositol (PI) with 18:0/20:5 and a compensative accumulation of putative PI(18:0/20:4) were detected in the fat-1 mutants. In conclusion, the whole-body MALDI-IMS technique is useful for the profiling of multiple biomolecules in C. elegans in both intra- and inter-individual levels. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 59 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 34% |
| Researcher | 10 | 17% |
| Student > Bachelor | 5 | 8% |
| Other | 5 | 8% |
| Student > Master | 3 | 5% |
| Other | 6 | 10% |
| Unknown | 10 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 22% |
| Agricultural and Biological Sciences | 10 | 17% |
| Chemistry | 10 | 17% |
| Medicine and Dentistry | 4 | 7% |
| Engineering | 3 | 5% |
| Other | 9 | 15% |
| Unknown | 10 | 17% |
Attention Score in Context
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#17,286,645
of 25,374,917 outputs
Outputs from Analytical & Bioanalytical Chemistry
#5,669
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Outputs of similar age
#167,418
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#50
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