| Title |
Enhancement of Surfactin yield by improving the medium composition and fermentation process
|
|---|---|
| Published in |
AMB Express, August 2015
|
| DOI | 10.1186/s13568-015-0145-0 |
| Pubmed ID | |
| Authors |
Judit Willenbacher, Wladimir Yeremchuk, Teresa Mohr, Christoph Syldatk, Rudolf Hausmann |
| Abstract |
Surfactin is one of the most promising biosurfactants due to its extraordinary surface activity. Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin. The current study investigated the enhancement of Surfactin yields by analyzing the effects of different glucose concentrations, next to the introduction of an alternative chelating agent and nitrogen source. The utilization of 8 g/L glucose, 0.008 mM Na3citrate and 50 mM (NH4)2SO4 increased Surfactin yields from 0.7 to 1.1 g/L during shake flask experiments applying Bacillus subtilis DSM10(T). Consequentially conducted shake flask experiments, employing five other Surfactin producer strains during cultivation in the former and enhanced version of the Cooper medium, suggest a general enhancement of Surfactin yields during application of the enhanced version of the Cooper medium. The enhancement of the medium composition is therefore most likely independent from the employed producer strain. The following utilization of the enhanced medium composition during fed-batch fermentation with integrated foam fractionation yielded 30 % more Surfactin in comparison to batch fermentations with integrated foam fractionation employing the former version of the Cooper medium. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 134 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | <1% |
| Denmark | 1 | <1% |
| Poland | 1 | <1% |
| Unknown | 131 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 16% |
| Student > Bachelor | 18 | 13% |
| Researcher | 16 | 12% |
| Student > Master | 16 | 12% |
| Student > Doctoral Student | 10 | 7% |
| Other | 22 | 16% |
| Unknown | 31 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 34 | 25% |
| Agricultural and Biological Sciences | 30 | 22% |
| Engineering | 8 | 6% |
| Chemistry | 6 | 4% |
| Immunology and Microbiology | 6 | 4% |
| Other | 13 | 10% |
| Unknown | 37 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2015.
All research outputs
#15,344,095
of 22,824,164 outputs
Outputs from AMB Express
#445
of 1,234 outputs
Outputs of similar age
#156,407
of 266,223 outputs
Outputs of similar age from AMB Express
#17
of 25 outputs
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