| Title |
Molecular cloning, expression and adhesion analysis of silent slpB of Lactobacillus acidophilus NCFM
|
|---|---|
| Published in |
AMB Express, June 2018
|
| DOI | 10.1186/s13568-018-0631-2 |
| Pubmed ID | |
| Authors |
Yuxing Guo, Xiangyue Li, Yao Yang, Zhen Wu, Xiaoqun Zeng, Fawze Nadari, Daodong Pan |
| Abstract |
The slpB gene of Lactobacillus acidophilus NCFM, which differs from the slpA gene and is silent under normal conditions, was successfully amplified and ligated to the corresponding available sites on a recombinant pET-28a vector. Then the pET-28a-slpB vector was transformed into Escherichia coli DH (DE3) and the fusion His-slpB protein was expressed by induction with 1 mM IPTG for 14 h at 37 °C. The resulting His-slpB protein (SB) had a relative molecular weight of 48 kDa. It was purified using a Ni-NTA column and was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot contrastive analysis. The slpA protein (SA) from L. acidophilus NCFM was extracted and purified. It had a relative molecular weight of 46 kDa. Circular dichroism measurements suggested that the two S-layer proteins had a high β-sheet content and a low α-helix structure content. In an adhesion experiment, SA displayed higher adhesive capability towards Caco-2 cells than did SB. The results suggest that these two S-layer proteins could have biotechnological applications. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 20% |
| Student > Doctoral Student | 2 | 20% |
| Professor | 2 | 20% |
| Lecturer | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 40% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Business, Management and Accounting | 1 | 10% |
| Chemistry | 1 | 10% |
| Unknown | 2 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2018.
All research outputs
#20,523,725
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Outputs from AMB Express
#976
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Outputs of similar age
#288,086
of 328,763 outputs
Outputs of similar age from AMB Express
#28
of 52 outputs
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