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Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica

Overview of attention for article published in AMB Express, August 2015
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About this Attention Score

  • Among the highest-scoring outputs from this source (#36 of 268)
  • Above-average Attention Score compared to outputs of the same age (62nd percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

twitter
3 tweeters

Citations

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5 Dimensions

Readers on

mendeley
24 Mendeley
Title
Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica
Published in
AMB Express, August 2015
DOI 10.1186/s13568-015-0139-y
Pubmed ID
Authors

Marcos H de Moraes, Max Teplitski

Abstract

Virulence plasmids borne by serovars of Salmonella enterica carry genes involved in its pathogenicity, as well as other functions. Characterization of phenotypes associated with virulence plasmids requires a system for efficiently curing strains of their virulence plasmids. Here, we developed a 3-step protocol for targeted curing of virulence plasmids. The protocol involves insertion of an I-SecI restriction site linked to an antibiotic resistance gene into the target plasmid using λ-Red mutagenesis, followed by the transformation with a temperature-sensitive auxiliary plasmid which carries I-SecI nuclease expressed from a tetracycline-inducible promoter. Finally, the auxiliary plasmid is removed by incubation at 42 °C and the plasmid-less strains are verified on antibiotic-containing media. This method is fast and very efficient: over 90 % of recovered colonies lacked their virulence plasmid.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Peru 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 29%
Student > Master 6 25%
Student > Bachelor 2 8%
Researcher 2 8%
Other 1 4%
Other 0 0%
Unknown 6 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 29%
Medicine and Dentistry 4 17%
Agricultural and Biological Sciences 3 13%
Immunology and Microbiology 2 8%
Veterinary Science and Veterinary Medicine 1 4%
Other 0 0%
Unknown 7 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2015.
All research outputs
#2,196,147
of 5,483,994 outputs
Outputs from AMB Express
#36
of 268 outputs
Outputs of similar age
#70,672
of 192,046 outputs
Outputs of similar age from AMB Express
#2
of 25 outputs
Altmetric has tracked 5,483,994 research outputs across all sources so far. This one has received more attention than most of these and is in the 59th percentile.
So far Altmetric has tracked 268 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 192,046 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.