| Title |
Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica
|
|---|---|
| Published in |
AMB Express, August 2015
|
| DOI | 10.1186/s13568-015-0139-y |
| Pubmed ID | |
| Authors |
Marcos H de Moraes, Max Teplitski |
| Abstract |
Virulence plasmids borne by serovars of Salmonella enterica carry genes involved in its pathogenicity, as well as other functions. Characterization of phenotypes associated with virulence plasmids requires a system for efficiently curing strains of their virulence plasmids. Here, we developed a 3-step protocol for targeted curing of virulence plasmids. The protocol involves insertion of an I-SecI restriction site linked to an antibiotic resistance gene into the target plasmid using λ-Red mutagenesis, followed by the transformation with a temperature-sensitive auxiliary plasmid which carries I-SecI nuclease expressed from a tetracycline-inducible promoter. Finally, the auxiliary plasmid is removed by incubation at 42 °C and the plasmid-less strains are verified on antibiotic-containing media. This method is fast and very efficient: over 90 % of recovered colonies lacked their virulence plasmid. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Peru | 1 | 4% |
| Unknown | 26 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 26% |
| Student > Master | 7 | 26% |
| Student > Bachelor | 2 | 7% |
| Researcher | 2 | 7% |
| Other | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 30% |
| Medicine and Dentistry | 4 | 15% |
| Agricultural and Biological Sciences | 3 | 11% |
| Immunology and Microbiology | 2 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 9 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2015.
All research outputs
#20,063,585
of 24,657,405 outputs
Outputs from AMB Express
#845
of 1,299 outputs
Outputs of similar age
#197,033
of 269,654 outputs
Outputs of similar age from AMB Express
#26
of 26 outputs
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