Title |
A microfluidic chip containing a molecularly imprinted polymer and a DNA aptamer for voltammetric determination of carbofuran
|
---|---|
Published in |
Microchimica Acta, May 2018
|
DOI | 10.1007/s00604-018-2835-1 |
Pubmed ID | |
Authors |
Shuhuai Li, Jianping Li, Jinhui Luo, Zhi Xu, Xionghui Ma |
Abstract |
An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV). The dual recognition (by aptamer and MIP) results in outstanding selectivity. Additionally, graphene oxide-supported gold nanoparticles (GO-AuNPs) were used to improve the sensitivity of electrochemical detector. DPV response is linear in the 0.2 to 50 nM carbofuran concentration range at a potential of -1.2 V, with a 67 pM detection limit. The method has attractive features such as its potential for high throughput, high degree of automation, and high integration. Conceivably, the method may be extended to other analytes for which appropriate MIPs and aptamers are available. Graphical abstract Schematic of an electrochemical microfluidic chip for carbofuran detection based on a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. In the chip, targets were recognized by MIP and aptamer, respectively. It shows promising potential for the design of electrochemical devices with high throughput, high automation, and high integration. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 61 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 18% |
Researcher | 8 | 13% |
Student > Doctoral Student | 6 | 10% |
Student > Master | 6 | 10% |
Student > Bachelor | 4 | 7% |
Other | 10 | 16% |
Unknown | 16 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 16 | 26% |
Biochemistry, Genetics and Molecular Biology | 8 | 13% |
Chemical Engineering | 3 | 5% |
Engineering | 3 | 5% |
Materials Science | 3 | 5% |
Other | 6 | 10% |
Unknown | 22 | 36% |