| Title |
An alkaline and surfactant-tolerant lipase from Trichoderma lentiforme ACCC30425 with high application potential in the detergent industry
|
|---|---|
| Published in |
AMB Express, June 2018
|
| DOI | 10.1186/s13568-018-0618-z |
| Pubmed ID | |
| Authors |
Yuzhou Wang, Rui Ma, Shigui Li, Mingbo Gong, Bin Yao, Yingguo Bai, Jingang Gu |
| Abstract |
Alkaline lipases with adaptability to low temperatures and strong surfactant tolerance are favorable for application in the detergent industry. In the present study, a lipase-encoding gene, TllipA, was cloned from Trichoderma lentiforme ACCC30425 and expressed in Pichia pastoris GS115. The purified recombinant TlLipA was found to have optimal activities at 50 °C and pH 9.5 and retain stable over the pH range of 6.0-10.0 and 40 °C and below. When using esters of different lengths as substrates, TlLipA showed preference for the medium length p-nitrophenyl octanoate. In comparison to commercial lipases, TlLipA demonstrated higher tolerance to various surfactants (SDS, Tween 20, and Triton X100) and retained more activities after incubation with Triton X100 for up to 24 h. These favorable characteristics make TlLipA prospective as an additive in the detergent industry. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 12 | 23% |
| Researcher | 6 | 12% |
| Student > Master | 5 | 10% |
| Student > Ph. D. Student | 3 | 6% |
| Student > Doctoral Student | 2 | 4% |
| Other | 9 | 17% |
| Unknown | 15 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 18 | 35% |
| Agricultural and Biological Sciences | 6 | 12% |
| Chemistry | 3 | 6% |
| Immunology and Microbiology | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 16 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 June 2018.
All research outputs
#20,520,426
of 23,088,369 outputs
Outputs from AMB Express
#975
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Outputs of similar age
#289,334
of 329,782 outputs
Outputs of similar age from AMB Express
#27
of 56 outputs
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