| Title |
In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence
|
|---|---|
| Published in |
AMB Express, May 2018
|
| DOI | 10.1186/s13568-018-0598-z |
| Pubmed ID | |
| Authors |
Jefferson Ivan Corrêa, Andreas Stocker, Soraya Castro Trindade, Vera Vale, Thais Brito, Bruno Bastos, José Tadeu Raynal, Patrícia Mares de Miranda, Adriano Costa de Alcantara, Songeli Menezes Freire, Lília Moura Costa, Roberto Meyer |
| Abstract |
Caseous lymphadenitis (LC) is a chronic contagious disease caused by Corynebacterium pseudotuberculosis, which mainly affects goats and sheep. Vaccination is an effective but not yet well-established method, partly due to a lack of knowledge surrounding the most effective immunoprotective components. The present study aimed to quantify and compare the in vivo expression of genes pld (phospholipase D), cpp (CP40), nanH (neuraminidase H), sodC (superoxide dismutase C) and spaC (adhesin) using qRT-PCR, with the respective expression in vitro. Caseous material of abscesses removed from five animals was cultured, with colonies suggestive of C. pseudotuberculosis identified. RNA extraction was performed on these samples, as well as on the respective pellets derived from liquid cultures brain heart infusion. After evaluating RNA integrity, complementary DNA was synthesized, followed by the relative quantification each of the genes of interest. Mean mRNA expression of the five genes found in abscesses and in cultures differed significantly, with respective values of: nanH 811.50 ± 198.27 and 359.35 ± 75.45 (p = 0.009); cpp 856.31 ± 385.11 and 154.54 ± 94.34 (p = 0.0039); plD 922.70 ± 450.73 and 212.41 ± 153.10 (p = 0.016); sodC 1,293.53 ± 564.75 and 223.63 ± 145.58 (p = 0.016); spaC 1,157.10 ± 525.13 and 214.26 ± 125.70 (p = 0,016). Expression was observed to be 6-8 times higher in abscesses than in cultures, Indicative that is a genetic expression of the in vitro bacterium exists, yet in vivo has a greater magnitude corroborating to one of these virulence factors in the pathogenesis of LC. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 27% |
| Student > Master | 9 | 24% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Postgraduate | 2 | 5% |
| Student > Doctoral Student | 1 | 3% |
| Other | 4 | 11% |
| Unknown | 7 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 6 | 16% |
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Agricultural and Biological Sciences | 6 | 16% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
| Immunology and Microbiology | 3 | 8% |
| Other | 4 | 11% |
| Unknown | 9 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2018.
All research outputs
#16,357,504
of 24,093,053 outputs
Outputs from AMB Express
#475
of 1,274 outputs
Outputs of similar age
#214,456
of 335,231 outputs
Outputs of similar age from AMB Express
#15
of 59 outputs
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