| Title |
Substrate stereoselectivity of poly(Asp) hydrolase-1 capable of cleaving β-amide bonds as revealed by investigation of enzymatic hydrolysis of stereoisomeric β-tri(Asp)s
|
|---|---|
| Published in |
AMB Express, June 2015
|
| DOI | 10.1186/s13568-015-0118-3 |
| Pubmed ID | |
| Authors |
Tomohiro Hiraishi, Hideki Abe, Mizuo Maeda |
| Abstract |
We previously reported that poly(Asp) hydrolase-1 (PahZ1KP-2) from Pedobacter sp. KP-2 selectively, but not completely, cleaved the amide bonds between β-Asp units in thermally synthesized poly(Asp) (tPAA). In the present study, the enzymatic hydrolysis of stereoisomeric β-tri(Asp)s by PahZ1KP-2 was investigated to clarify the substrate stereoselectivity of PahZ1KP-2 in the hydrolysis of tPAA. The results suggest the following structural features of PahZ1KP-2 at its substrate binding site: (1) the active site contains four subsites (2, 1, -1, and -2), three of which need to be occupied by Asp units for cleavage to occur; (2) for the hydrolysis to proceed, subsite 1 should be occupied by an L-Asp unit, whereas the other three subsites may accept both L- and D-Asp units; (3) for the two central subsites between which cleavage occurs, the (L-Asp)-(D-Asp) sequence is the most favorable for cleavage. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 27% |
| Unspecified | 1 | 9% |
| Lecturer > Senior Lecturer | 1 | 9% |
| Professor > Associate Professor | 1 | 9% |
| Unknown | 5 | 45% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 9% |
| Environmental Science | 1 | 9% |
| Biochemistry, Genetics and Molecular Biology | 1 | 9% |
| Agricultural and Biological Sciences | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 5 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 June 2015.
All research outputs
#15,336,434
of 22,811,321 outputs
Outputs from AMB Express
#445
of 1,234 outputs
Outputs of similar age
#156,867
of 267,086 outputs
Outputs of similar age from AMB Express
#10
of 15 outputs
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