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Spatio-temporal dynamics of calcium electrotransfer during cell membrane permeabilization

Overview of attention for article published in Drug Delivery and Translational Research, May 2018
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#4 of 164)
  • High Attention Score compared to outputs of the same age (94th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

news
5 news outlets
blogs
1 blog
twitter
3 tweeters

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
8 Mendeley
Title
Spatio-temporal dynamics of calcium electrotransfer during cell membrane permeabilization
Published in
Drug Delivery and Translational Research, May 2018
DOI 10.1007/s13346-018-0533-5
Pubmed ID
Authors

Alexis Guionet, S. Moosavi Nejad, Justin Teissié, Takashi Sakugawa, Sunao Katsuki, Hidenori Akiyama, Hamid Hosseini

Abstract

Pulsed electric fields (PEFs) are applied as physical stimuli for DNA/drug delivery, cancer therapy, gene transformation, and microorganism eradication. Meanwhile, calcium electrotransfer offers an interesting approach to treat cancer, as it induces cell death easier in malignant cells than in normal cells. Here, we study the spatial and temporal cellular responses to 10 μs duration PEFs; by observing real-time, the uptake of extracellular calcium through the cell membrane. The experimental setup consisted of an inverted fluorescence microscope equipped with a color high-speed framing camera and a specifically designed miniaturized pulsed power system. The setup allowed us to accurately observe the permeabilization of HeLa S3 cells during application of various levels of PEFs ranging from 0.27 to 1.80 kV/cm. The low electric field experiments confirmed the threshold value of transmembrane potential (TMP). The high electric field observations enabled us to retrieve the entire spatial variation of the permeabilization angle (θ). The temporal observations proved that after a minimal permeabilization of the cell membrane, the ionic diffusion was the prevailing mechanism of the delivery to the cell cytoplasm. The observations suggest 0.45 kV/cm and 100 pulses at 1 kHz as an optimal condition to achieve full calcium concentration in the cell cytoplasm. The results offer precise levels of electric fields to control release of the extracellular calcium to the cell cytoplasm for inducing minimally invasive cancer calcium electroporation, an interesting affordable method to treat cancer patients with minimum side effects.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 25%
Student > Ph. D. Student 2 25%
Unspecified 1 13%
Professor 1 13%
Other 1 13%
Other 1 13%
Readers by discipline Count As %
Engineering 2 25%
Biochemistry, Genetics and Molecular Biology 2 25%
Physics and Astronomy 1 13%
Unspecified 1 13%
Unknown 2 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 48. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 August 2018.
All research outputs
#355,109
of 13,350,484 outputs
Outputs from Drug Delivery and Translational Research
#4
of 164 outputs
Outputs of similar age
#14,965
of 269,198 outputs
Outputs of similar age from Drug Delivery and Translational Research
#3
of 9 outputs
Altmetric has tracked 13,350,484 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 164 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,198 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.