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Cryopreservation without vitrification suitable for large scale cryopreservation of orchid seeds

Overview of attention for article published in Botanical Studies, May 2018
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  • Among the highest-scoring outputs from this source (#33 of 163)
  • Above-average Attention Score compared to outputs of the same age (64th percentile)

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28 Mendeley
Cryopreservation without vitrification suitable for large scale cryopreservation of orchid seeds
Published in
Botanical Studies, May 2018
DOI 10.1186/s40529-018-0229-7
Pubmed ID

Emily Schofield, Edward P. Jones, Viswambharan Sarasan


Orchids are under threat from human activities and climate change, with populations limited to small geographic hotspots. This makes them ideal candidates for ex situ conservation. Orchid seeds are desiccation tolerant, but often have poor longevity in seed banks and cryopreservation of orchid protocorms is complex and expensive. Therefore, simple methods for large-scale storage programs are essential to store orchid seeds of different life forms. Seeds of five species representing epiphytic, lithophytic and terrestrial orchids from the Central Highlands of Madagascar were studied to find a simple and effective system of cryopreservation. The use of a vitrification solution prior to cryopreservation to improve survival was investigated, as well as the use of symbiotic and asymbiotic germination media to maximise germination after cryopreservation. Using the filter paper packet method, dried seeds were stored in vapour phase above liquid nitrogen and recovered after thawing with both symbiotic and asymbiotic media. The study revealed that cryoprotection is not essential for the species in this study, which represented a range of lifeforms. Vitrification generally led to a decrease in germination post cryopreservation. The use of a symbiotic germination medium post cryopreservation was found to be successful in the species in which it was tested. However, the use of an asymbiotic medium was successful for all the species in this study. Vitrification was not essential for the species in this study as the orchid seeds were already ultralow temperature and desiccation tolerant. However, further studies using more species are required to validate this approach. This may be an ecophysiological or genetic trait of these species. Therefore, this form of dry seed cryopreservation could form part of ex situ orchid seed conservation using a standard method. The methods developed here will store greater genetic diversity compared to what can be achieved with protocorms and are suitable for both asymbiotic and symbiotic recovery after cryopreservation. This will help reduce the time and cost of ex situ conservation, and help develop universal protocols for large genera, compared to custom protocols required for protocorm cryopreservation.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 25%
Student > Master 4 14%
Researcher 3 11%
Student > Doctoral Student 2 7%
Student > Bachelor 2 7%
Other 7 25%
Unknown 3 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 46%
Social Sciences 3 11%
Business, Management and Accounting 2 7%
Environmental Science 1 4%
Biochemistry, Genetics and Molecular Biology 1 4%
Other 3 11%
Unknown 5 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2018.
All research outputs
of 22,202,663 outputs
Outputs from Botanical Studies
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Outputs of similar age
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Outputs of similar age from Botanical Studies
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Altmetric has tracked 22,202,663 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 163 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,127 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them