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[18F]FDG-6-P as a novel in vivo tool for imaging staphylococcal infections

Overview of attention for article published in EJNMMI Research, March 2015
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Title
[18F]FDG-6-P as a novel in vivo tool for imaging staphylococcal infections
Published in
EJNMMI Research, March 2015
DOI 10.1186/s13550-015-0095-1
Pubmed ID
Authors

Bethany Mills, Ramla O Awais, Jeni Luckett, Dave Turton, Paul Williams, Alan C Perkins, Philip J Hill

Abstract

Management of infection is a major clinical problem. Staphylococcus aureus is a Gram-positive bacterium which colonises approximately one third of the adult human population. Staphylococcal infections can be life-threatening and are frequently complicated by multi-antibiotic resistant strains including methicillin-resistant S. aureus (MRSA). Fluorodeoxyglucose ([(18)F]FDG) imaging has been used to identify infection sites; however, it is unable to distinguish between sterile inflammation and bacterial load. We have modified [(18)F]FDG by phosphorylation, producing [(18)F]FDG-6-P to facilitate specific uptake and accumulation by S. aureus through hexose phosphate transporters, which are not present in mammalian cell membranes. This approach leads to the specific uptake of the radiopharmaceutical into the bacteria and not the sites of sterile inflammation. [(18)F]FDG-6-P was synthesised from [(18)F]FDG. Yield, purity and stability were confirmed by RP-HPLC and iTLC. The specificity of [(18)F]FDG-6-P for the bacterial universal hexose phosphate transporter (UHPT) was confirmed with S. aureus and mammalian cell assays in vitro. Whole body biodistribution and accumulation of [(18)F]FDG-6-P at the sites of bioluminescent staphylococcal infection were established in a murine foreign body infection model. In vitro validation assays demonstrated that [(18)F]FDG-6-P was stable and specifically transported into S. aureus but not mammalian cells. [(18)F]FDG-6-P was elevated at the sites of S. aureus infection in vivo compared to uninfected controls; however, the increase in signal was not significant and unexpectedly, the whole-body biodistribution of [(18)F]FDG-6-P was similar to that of [(18)F]FDG. Despite conclusive in vitro validation, [(18)F]FDG-6-P did not behave as predicted in vivo. However at the site of known infection, [(18)F]FDG-6-P levels were elevated compared with uninfected controls, providing a higher signal-to-noise ratio. The bacterial UHPT can transport hexose phosphates other than glucose, and therefore alternative sugars may show differential biodistribution and provide a means for specific bacterial detection.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 3%
Unknown 32 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 24%
Researcher 7 21%
Student > Ph. D. Student 4 12%
Student > Postgraduate 3 9%
Student > Bachelor 2 6%
Other 7 21%
Unknown 2 6%
Readers by discipline Count As %
Medicine and Dentistry 8 24%
Agricultural and Biological Sciences 6 18%
Pharmacology, Toxicology and Pharmaceutical Science 4 12%
Chemistry 4 12%
Biochemistry, Genetics and Molecular Biology 3 9%
Other 6 18%
Unknown 2 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2015.
All research outputs
#20,273,512
of 22,805,349 outputs
Outputs from EJNMMI Research
#385
of 556 outputs
Outputs of similar age
#223,252
of 263,705 outputs
Outputs of similar age from EJNMMI Research
#14
of 17 outputs
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So far Altmetric has tracked 556 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.