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Quantification of cell fusion events human breast cancer cells and breast epithelial cells using a Cre-LoxP-based double fluorescence reporter system

Overview of attention for article published in Cellular & Molecular Life Sciences, April 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (72nd percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

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8 tweeters

Citations

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12 Dimensions

Readers on

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21 Mendeley
Title
Quantification of cell fusion events human breast cancer cells and breast epithelial cells using a Cre-LoxP-based double fluorescence reporter system
Published in
Cellular & Molecular Life Sciences, April 2015
DOI 10.1007/s00018-015-1910-6
Pubmed ID
Authors

Marieke Mohr, Songül Tosun, Wolfgang H. Arnold, Frank Edenhofer, Kurt S. Zänker, Thomas Dittmar

Abstract

The biological phenomenon of cell fusion plays an important role in several physiological processes, like fertilization, placentation, or wound healing/tissue regeneration, as well as pathophysiological processes, such as cancer. Despite this fact, considerably less is still known about the factors and conditions that will induce the merging of two plasma membranes. Inflammation and proliferation has been suggested as a positive trigger for cell fusion, but it remains unclear, which of the factor(s) of the inflamed microenvironment are being involved. To clarify this we developed a reliable assay to quantify the in vitro fusion frequency of cells using a fluorescence double reporter vector (pFDR) containing a LoxP-flanked HcRed/DsRed expression cassette followed by an EGFP expression cassette. Because cell fusion has been implicated in cancer progression four human breast cancer cell lines were stably transfected with a pFDR vector and were co-cultured with the stably Cre-expressing human breast epithelial cell line. Cell fusion is associated with a Cre-mediated recombination resulting in induction of EGFP expression in hybrid cells, which can be quantified by flow cytometry. By testing a panel of different cytokines, chemokines, growth factors and other compounds, including exosomes, under normoxic and hypoxic conditions our data indicate that the proinflammatory cytokine TNF-α together with hypoxia is a strong inducer of cell fusion in human MDA-MB-435 and MDA-MB-231 breast cancer cells.

Twitter Demographics

The data shown below were collected from the profiles of 8 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 33%
Researcher 4 19%
Professor 2 10%
Student > Doctoral Student 2 10%
Student > Bachelor 2 10%
Other 4 19%
Readers by discipline Count As %
Medicine and Dentistry 7 33%
Agricultural and Biological Sciences 6 29%
Biochemistry, Genetics and Molecular Biology 4 19%
Neuroscience 2 10%
Social Sciences 1 5%
Other 1 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2017.
All research outputs
#3,175,421
of 12,281,604 outputs
Outputs from Cellular & Molecular Life Sciences
#628
of 2,445 outputs
Outputs of similar age
#63,455
of 233,748 outputs
Outputs of similar age from Cellular & Molecular Life Sciences
#8
of 64 outputs
Altmetric has tracked 12,281,604 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 2,445 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 233,748 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.