| Title |
Salinity effect on the metabolic pathway and microbial function in phenanthrene degradation by a halophilic consortium
|
|---|---|
| Published in |
AMB Express, April 2018
|
| DOI | 10.1186/s13568-018-0594-3 |
| Pubmed ID | |
| Authors |
Chongyang Wang, Yong Huang, Zuotao Zhang, Hui Wang |
| Abstract |
With the close relationship between saline environments and industry, polycyclic aromatic hydrocarbons (PAHs) accumulate in saline/hypersaline environments. Therefore, PAHs degradation by halotolerant/halophilic bacteria has received increasing attention. In this study, the metabolic pathway of phenanthrene degradation by halophilic consortium CY-1 was first studied which showed a single upstream pathway initiated by dioxygenation at the C1 and C2 positions, and at several downstream pathways, including the catechol pathway, gentisic acid pathway and protocatechuic acid pathway. The effects of salinity on the community structure and expression of catabolic genes were further studied by a combination of high-throughput sequencing, catabolic gene clone library and real-time PCR. Pure cultures were also isolated from consortium CY-1 to investigate the contribution made by different microbes in the PAH-degrading process. Marinobacter is the dominant genus that contributed to the upstream degradation of phenanthrene especially in high salt content. Genus Halomonas made a great contribution in transforming intermediates in the subsequent degradation of catechol by using catechol 1,2-dioxygenase (C12O). Other microbes were predicted to be mediating bacteria that were able to utilize intermediates via different downstream pathways. Salinity was investigated to have negative effects on both microbial diversity and activity of consortium CY-1 and consortium CY-1 was found with a high degree of functional redundancy in saline environments. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 20% |
| Student > Master | 5 | 12% |
| Researcher | 5 | 12% |
| Other | 3 | 7% |
| Student > Bachelor | 3 | 7% |
| Other | 5 | 12% |
| Unknown | 12 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 17% |
| Environmental Science | 7 | 17% |
| Biochemistry, Genetics and Molecular Biology | 3 | 7% |
| Chemical Engineering | 3 | 7% |
| Immunology and Microbiology | 3 | 7% |
| Other | 5 | 12% |
| Unknown | 13 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2018.
All research outputs
#20,483,282
of 23,045,021 outputs
Outputs from AMB Express
#974
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Outputs of similar age
#287,576
of 326,539 outputs
Outputs of similar age from AMB Express
#35
of 59 outputs
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