| Title |
Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose
|
|---|---|
| Published in |
AMB Express, February 2015
|
| DOI | 10.1186/s13568-015-0100-0 |
| Pubmed ID | |
| Authors |
Anders G Sandström, Alejandro Muñoz de las Heras, Diogo Portugal-Nunes, Marie F Gorwa-Grauslund |
| Abstract |
Poly-3-d-hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker's yeast Saccharomyces cerevisiae was engineered to produce PHB from xylose, the main pentose found in lignocellulosic biomass. The PHB pathway genes from the well-characterized PHB producer Cupriavidus necator were introduced in recombinant S. cerevisiae strains already capable of pentose utilization by introduction of the fungal genes for xylose utilization from the yeast Scheffersomyces stipitis. PHB production from xylose was successfully demonstrated in shake-flasks experiments, with PHB yield of 1.17 ± 0.18 mg PHB g(-1) xylose. Under well-controlled fully aerobic conditions, a titer of 101.7 mg PHB L(-1) was reached within 48 hours, with a PHB yield of 1.99 ± 0.15 mg PHB g(-1) xylose, thereby demonstrating the potential of this host for PHB production from lignocellulose. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 88 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 16 | 18% |
| Student > Bachelor | 15 | 17% |
| Researcher | 14 | 16% |
| Student > Ph. D. Student | 11 | 13% |
| Student > Doctoral Student | 5 | 6% |
| Other | 11 | 13% |
| Unknown | 16 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 30% |
| Agricultural and Biological Sciences | 20 | 23% |
| Chemical Engineering | 6 | 7% |
| Immunology and Microbiology | 5 | 6% |
| Engineering | 5 | 6% |
| Other | 5 | 6% |
| Unknown | 21 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 March 2022.
All research outputs
#6,481,449
of 23,400,864 outputs
Outputs from AMB Express
#115
of 1,255 outputs
Outputs of similar age
#71,998
of 256,612 outputs
Outputs of similar age from AMB Express
#1
of 18 outputs
Altmetric has tracked 23,400,864 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,255 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 256,612 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.