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Genetic and physiological basis for antibody production by Kluyveromyces marxianus

Overview of attention for article published in AMB Express, April 2018
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  • Good Attention Score compared to outputs of the same age and source (70th percentile)

Mentioned by

twitter
4 tweeters

Citations

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3 Dimensions

Readers on

mendeley
20 Mendeley
Title
Genetic and physiological basis for antibody production by Kluyveromyces marxianus
Published in
AMB Express, April 2018
DOI 10.1186/s13568-018-0588-1
Pubmed ID
Authors

Yumiko Nambu-Nishida, Keiji Nishida, Tomohisa Hasunuma, Akihiko Kondo

Abstract

Kluyveromyces marxianus is a thermotolerant, crabtree-negative yeast, which preferentially directs metabolism (e.g., from the tricarboxylic acid cycle) to aerobic alcoholic fermentation. Thus K. marxianus has great potential for engineering to produce various materials under aerobic cultivation conditions. In this study, we engineered K. marxianus to produce and secrete a single-chain antibody (scFv), a product that is highly valuable but has historically proven difficult to generate at large scale. scFv production was obtained with strains carrying either plasmid-borne or genomically integrated constructs using various combinations of promoters (P MDH1 or P ACO1 ) and secretion signal peptides (KmINUss or Scα-MFss). As the wild-type K. marxianus secretes endogenous inulinase predominantly, the corresponding INU1 gene was disrupted using a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated protein (CRISPR-Cas9) system to re-direct resources to scFv production. Genomic integration was used to replace INU1 with sequences encoding a fusion of the INU1 signal peptide to scFv; the resulting construct yielded the highest scFv production among the strains tested. Optimization of growth conditions revealed that scFv production by this strain was enhanced by incubation at 30 °C in xylose medium containing 200 mM MgSO4. These results together demonstrate that K. marxianus has the potential to serve as a host strain for antibody production.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 20%
Student > Master 3 15%
Other 2 10%
Professor > Associate Professor 2 10%
Student > Ph. D. Student 2 10%
Other 4 20%
Unknown 3 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 45%
Agricultural and Biological Sciences 6 30%
Medicine and Dentistry 1 5%
Unknown 4 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 April 2018.
All research outputs
#7,385,106
of 12,801,247 outputs
Outputs from AMB Express
#197
of 804 outputs
Outputs of similar age
#139,940
of 271,765 outputs
Outputs of similar age from AMB Express
#8
of 30 outputs
Altmetric has tracked 12,801,247 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 804 research outputs from this source. They receive a mean Attention Score of 2.4. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,765 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.