Title |
CLE14/CLE20 peptides may interact with CLAVATA2/CORYNE receptor-like kinases to irreversibly inhibit cell division in the root meristem of Arabidopsis
|
---|---|
Published in |
Planta, August 2010
|
DOI | 10.1007/s00425-010-1236-4 |
Pubmed ID | |
Authors |
Ling Meng, Lewis J. Feldman |
Abstract |
Towards an understanding of the interacting nature of the CLAVATA (CLV) complex, we predicted the 3D structures of CLV3/ESR-related (CLE) peptides and the ectodomain of their potential receptor proteins/kinases, and docking models of these molecules. The results show that the ectodomain of CLV1 can form homodimers and that the 12-/13-amino-acid CLV3 peptide fits into the binding clefts of the CLV1 dimers. Our results also demonstrate that the receptor domain of CORYNE (CRN), a recently identified receptor-like kinase, binds tightly to the ectodomain of CLV2, and this likely leads to an increased possibility for docking with CLV1. Furthermore, our docking models reveal that two CRN-CLV2 ectodomain heterodimers are able to form a tetramer receptor complex. Peptides of CLV3, CLE14, CLE19, and CLE20 are also able to bind a potential CLV2-CRN heterodimer or heterotetramer complex. Using a cell-division reporter line, we found that synthetic 12-amino-acid CLE14 and CLE20 peptides inhibit, irreversibly, root growth by reducing cell division rates in the root apical meristem, resulting in a short-root phenotype. Intriguingly, we observed that exogenous application of cytokinin can partially rescue the short-root phenotype induced by over-expression of either CLE14 or CLE20 in planta. However, cytokinin treatment does not rescue the short-root phenotype caused by exogenous application of the synthetic CLE14/CLE20 peptides, suggesting a requirement for a condition provided only in living plants. These results therefore imply that the CLE14/CLE20 peptides may act through the CLV2-CRN receptor kinase, and that their availabilities and/or abundances may be affected by cytokinin activity in planta. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Australia | 2 | 2% |
United States | 2 | 2% |
Czechia | 1 | 1% |
Germany | 1 | 1% |
Spain | 1 | 1% |
China | 1 | 1% |
Unknown | 88 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 22% |
Researcher | 21 | 22% |
Student > Master | 12 | 13% |
Student > Bachelor | 7 | 7% |
Student > Doctoral Student | 7 | 7% |
Other | 14 | 15% |
Unknown | 14 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 64 | 67% |
Biochemistry, Genetics and Molecular Biology | 12 | 13% |
Computer Science | 2 | 2% |
Psychology | 1 | 1% |
Engineering | 1 | 1% |
Other | 0 | 0% |
Unknown | 16 | 17% |