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Isopropanol production with engineered Cupriavidus necator as bioproduction platform

Overview of attention for article published in Applied Microbiology & Biotechnology, March 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#37 of 5,579)
  • High Attention Score compared to outputs of the same age (93rd percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
patent
1 patent
wikipedia
1 Wikipedia page

Citations

dimensions_citation
49 Dimensions

Readers on

mendeley
108 Mendeley
Title
Isopropanol production with engineered Cupriavidus necator as bioproduction platform
Published in
Applied Microbiology & Biotechnology, March 2014
DOI 10.1007/s00253-014-5591-0
Pubmed ID
Authors

Estelle Grousseau, Jingnan Lu, Nathalie Gorret, Stéphane E. Guillouet, Anthony J. Sinskey

Abstract

Alleviating our society's dependence on petroleum-based chemicals has been highly emphasized due to fossil fuel shortages and increasing greenhouse gas emissions. Isopropanol is a molecule of high potential to replace some petroleum-based chemicals, which can be produced through biological platforms from renewable waste carbon streams such as carbohydrates, fatty acids, or CO2. In this study, for the first time, the heterologous expression of engineered isopropanol pathways were evaluated in a Cupriavidus necator strain Re2133, which was incapable of producing poly-3-hydroxybutyrate [P(3HB)]. These synthetic production pathways were rationally designed through codon optimization, gene placement, and gene dosage in order to efficiently divert carbon flow from P(3HB) precursors toward isopropanol. Among the constructed pathways, Re2133/pEG7c overexpressing native C. necator genes encoding a β-ketothiolase, a CoA-transferase, and codon-optimized Clostridium genes encoding an acetoacetate decarboxylase and an alcohol dehydrogenase produced up to 3.44 g l(-1) isopropanol in batch culture, from fructose as a sole carbon source, with only 0.82 g l(-1) of biomass. The intrinsic performance of this strain (maximum specific production rate 0.093 g g(-1) h(-1), yield 0.32 Cmole Cmole(-1)) corresponded to more than 60 % of the respective theoretical performance. Moreover, the overall isopropanol production yield (0.24 Cmole Cmole(-1)) and the overall specific productivity (0.044 g g(-1) h(-1)) were higher than the values reported in the literature to date for heterologously engineered isopropanol production strains in batch culture. Strain Re2133/pEG7c presents good potential for scale-up production of isopropanol from various substrates in high cell density cultures.

Mendeley readers

The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 3 3%
India 2 2%
Germany 1 <1%
China 1 <1%
Unknown 101 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 33 31%
Student > Master 22 20%
Researcher 21 19%
Unspecified 13 12%
Student > Bachelor 7 6%
Other 12 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 38 35%
Biochemistry, Genetics and Molecular Biology 28 26%
Unspecified 18 17%
Engineering 7 6%
Chemistry 7 6%
Other 10 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 20. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2019.
All research outputs
#846,881
of 13,726,653 outputs
Outputs from Applied Microbiology & Biotechnology
#37
of 5,579 outputs
Outputs of similar age
#19,665
of 282,424 outputs
Outputs of similar age from Applied Microbiology & Biotechnology
#1
of 126 outputs
Altmetric has tracked 13,726,653 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,579 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,424 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.