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Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation

Overview of attention for article published in Applied Microbiology & Biotechnology, August 1997
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

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7 patents
wikipedia
1 Wikipedia page

Citations

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147 Dimensions

Readers on

mendeley
76 Mendeley
Title
Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation
Published in
Applied Microbiology & Biotechnology, August 1997
DOI 10.1007/s002530051041
Pubmed ID
Authors

M. Walfridsson, M. Anderlund, X. Bao, B. Hahn-Hägerdal

Abstract

Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yeast vector YEp24. Different vector constructions were made resulting in different specific activities of XR and XDH. The XR:XDH ratio (ratio of specific enzyme activities) of the transformed S. cerevisiae strains varied from 17.5 to 0.06. In order to enhance xylose utilisation in the XYL1-, XYL2-containing S. cerevisiae strains, the native genes encoding transketolase and transaldolase were also overexpressed. A strain with an XR:XDH ratio of 17.5 formed 0.82 g xylitol/g consumed xylose, whereas a strain with an XR:XDH ratio of 5.0 formed 0.58 g xylitol/g xylose. The strain with an XR:XDH ratio of 0.06, on the other hand, formed no xylitol and less glycerol and acetic acid compared with strains with the higher XR:XDH ratios. In addition, the strain with an XR:XDH ratio of 0.06 produced more ethanol than the other strains.

Mendeley readers

The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 1%
United States 1 1%
Brazil 1 1%
Unknown 73 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 23 30%
Student > Ph. D. Student 20 26%
Student > Master 12 16%
Student > Bachelor 8 11%
Student > Postgraduate 4 5%
Other 9 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 53%
Biochemistry, Genetics and Molecular Biology 13 17%
Engineering 12 16%
Chemistry 6 8%
Immunology and Microbiology 2 3%
Other 2 3%
Unknown 1 1%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2016.
All research outputs
#1,192,400
of 10,145,699 outputs
Outputs from Applied Microbiology & Biotechnology
#124
of 3,675 outputs
Outputs of similar age
#37,744
of 252,874 outputs
Outputs of similar age from Applied Microbiology & Biotechnology
#7
of 128 outputs
Altmetric has tracked 10,145,699 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,675 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,874 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 128 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.