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Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation

Overview of attention for article published in Applied Microbiology and Biotechnology, August 1997
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

Mentioned by

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7 patents
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2 Wikipedia pages

Citations

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171 Dimensions

Readers on

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90 Mendeley
Title
Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation
Published in
Applied Microbiology and Biotechnology, August 1997
DOI 10.1007/s002530051041
Pubmed ID
Authors

M. Walfridsson, M. Anderlund, X. Bao, B. Hahn-Hägerdal

Abstract

Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yeast vector YEp24. Different vector constructions were made resulting in different specific activities of XR and XDH. The XR:XDH ratio (ratio of specific enzyme activities) of the transformed S. cerevisiae strains varied from 17.5 to 0.06. In order to enhance xylose utilisation in the XYL1-, XYL2-containing S. cerevisiae strains, the native genes encoding transketolase and transaldolase were also overexpressed. A strain with an XR:XDH ratio of 17.5 formed 0.82 g xylitol/g consumed xylose, whereas a strain with an XR:XDH ratio of 5.0 formed 0.58 g xylitol/g xylose. The strain with an XR:XDH ratio of 0.06, on the other hand, formed no xylitol and less glycerol and acetic acid compared with strains with the higher XR:XDH ratios. In addition, the strain with an XR:XDH ratio of 0.06 produced more ethanol than the other strains.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 1%
Brazil 1 1%
Unknown 88 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 25 28%
Student > Ph. D. Student 21 23%
Student > Master 15 17%
Student > Bachelor 9 10%
Student > Postgraduate 3 3%
Other 10 11%
Unknown 7 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 39 43%
Biochemistry, Genetics and Molecular Biology 16 18%
Engineering 13 14%
Chemistry 6 7%
Immunology and Microbiology 3 3%
Other 4 4%
Unknown 9 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 February 2016.
All research outputs
#3,798,611
of 25,373,627 outputs
Outputs from Applied Microbiology and Biotechnology
#589
of 8,290 outputs
Outputs of similar age
#2,198
of 28,123 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#1
of 28 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,290 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 28,123 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.