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Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Overview of attention for article published in AMB Express, August 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#42 of 1,096)
  • High Attention Score compared to outputs of the same age (84th percentile)

Mentioned by

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2 patents
wikipedia
1 Wikipedia page

Citations

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66 Dimensions

Readers on

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106 Mendeley
Title
Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains
Published in
AMB Express, August 2012
DOI 10.1186/2191-0855-2-45
Pubmed ID
Authors

Florent Collas, Wouter Kuit, Benjamin Clément, Rémy Marchal, Ana M López-Contreras, Frederic Monot

Abstract

Isopropanol represents a widely-used commercial alcohol which is currently produced from petroleum. In nature, isopropanol is excreted by some strains of Clostridium beijerinckii, simultaneously with butanol and ethanol during the isopropanol butanol ethanol (IBE) fermentation. In order to increase isopropanol production, the gene encoding the secondary-alcohol dehydrogenase enzyme from C. beijerinckii NRRL B593 (adh) which catalyzes the reduction of acetone to isopropanol, was cloned into the acetone, butanol and ethanol (ABE)-producing strain C. acetobutylicum ATCC 824. The transformants showed high capacity for conversion of acetone into isopropanol (> 95%). To increase isopropanol production levels in ATCC 824, polycistronic transcription units containing, in addition to the adh gene, homologous genes of the acetoacetate decarboxylase (adc), and/or the acetoacetyl-CoA:acetate/butyrate:CoA transferase subunits A and B (ctfA and ctfB) were constructed and introduced into the wild-type strain. Combined overexpression of the ctfA and ctfB genes resulted in enhanced solvent production. In non-pH-controlled batch cultures, the total solvents excreted by the transformant overexpressing the adh, ctfA, ctfB and adc genes were 24.4 g/L IBE (including 8.8 g/L isopropanol), while the control strain harbouring an empty plasmid produced only 20.2 g/L ABE (including 7.6 g/L acetone). The overexpression of the adc gene had limited effect on IBE production. Interestingly, all transformants with the adh gene converted acetoin (a minor fermentation product) into 2,3-butanediol, highlighting the wide metabolic versatility of solvent-producing Clostridia.

Mendeley readers

The data shown below were compiled from readership statistics for 106 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
New Zealand 1 <1%
Colombia 1 <1%
France 1 <1%
Brazil 1 <1%
Unknown 102 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 27%
Student > Master 24 23%
Researcher 17 16%
Student > Bachelor 13 12%
Professor > Associate Professor 6 6%
Other 13 12%
Unknown 4 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 47 44%
Biochemistry, Genetics and Molecular Biology 23 22%
Engineering 13 12%
Chemical Engineering 8 8%
Chemistry 3 3%
Other 6 6%
Unknown 6 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 June 2020.
All research outputs
#2,543,970
of 18,034,577 outputs
Outputs from AMB Express
#42
of 1,096 outputs
Outputs of similar age
#43,958
of 298,745 outputs
Outputs of similar age from AMB Express
#1
of 1 outputs
Altmetric has tracked 18,034,577 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,096 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,745 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them