| Title |
A high-content chemical screen identifies ellipticine as a modulator of p53 nuclear localization
|
|---|---|
| Published in |
Apoptosis, January 2008
|
| DOI | 10.1007/s10495-007-0175-4 |
| Pubmed ID | |
| Authors |
G. Wei Xu, Imtiaz A. Mawji, Chloe J. Macrae, C. Anne Koch, Alessandro Datti, Jeffrey L. Wrana, James W. Dennis, Aaron D. Schimmer |
| Abstract |
p53 regulates apoptosis and the cell cycle through actions in the nucleus and cytoplasm. Altering the subcellular localization of p53 can alter its biological function. Therefore, small molecules that change the localization of p53 would be useful chemical probes to understand the influence of subcellular localization on the function of p53. To identify such molecules, a high-content screen for compounds that increased the localization of p53 to the nucleus or cytoplasm was developed, automated, and conducted. With this image-based assay, we identified ellipticine that increased the nuclear localization of GFP-mutant p53 protein but not GFP alone in Saos-2 osteosarcoma cells. In addition, ellipticine increased the nuclear localization of endogenous p53 in HCT116 colon cancer cells with a resultant increase in the transactivation of the p21 promoter. Increased nuclear p53 after ellipticine treatment was not associated with an increase in DNA double stranded breaks, indicating that ellipticine shifts p53 to the nucleus through a mechanism independent of DNA damage. Thus, a chemical biology approach has identified a molecule that shifts the localization of p53 and enhances its nuclear activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 5% |
| United States | 1 | 3% |
| Canada | 1 | 3% |
| Unknown | 34 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 26% |
| Student > Ph. D. Student | 7 | 18% |
| Student > Bachelor | 5 | 13% |
| Other | 3 | 8% |
| Student > Postgraduate | 3 | 8% |
| Other | 8 | 21% |
| Unknown | 2 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 24% |
| Biochemistry, Genetics and Molecular Biology | 9 | 24% |
| Chemistry | 6 | 16% |
| Medicine and Dentistry | 4 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
| Other | 4 | 11% |
| Unknown | 3 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2020.
All research outputs
#4,696,232
of 22,786,691 outputs
Outputs from Apoptosis
#70
of 804 outputs
Outputs of similar age
#22,108
of 156,129 outputs
Outputs of similar age from Apoptosis
#1
of 8 outputs
Altmetric has tracked 22,786,691 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 804 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 156,129 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them