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A high-content chemical screen identifies ellipticine as a modulator of p53 nuclear localization

Overview of attention for article published in Apoptosis, January 2008
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

patent
3 patents

Citations

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37 Dimensions

Readers on

mendeley
30 Mendeley
Title
A high-content chemical screen identifies ellipticine as a modulator of p53 nuclear localization
Published in
Apoptosis, January 2008
DOI 10.1007/s10495-007-0175-4
Pubmed ID
Authors

G. Wei Xu, Imtiaz A. Mawji, Chloe J. Macrae, C. Anne Koch, Alessandro Datti, Jeffrey L. Wrana, James W. Dennis, Aaron D. Schimmer

Abstract

p53 regulates apoptosis and the cell cycle through actions in the nucleus and cytoplasm. Altering the subcellular localization of p53 can alter its biological function. Therefore, small molecules that change the localization of p53 would be useful chemical probes to understand the influence of subcellular localization on the function of p53. To identify such molecules, a high-content screen for compounds that increased the localization of p53 to the nucleus or cytoplasm was developed, automated, and conducted. With this image-based assay, we identified ellipticine that increased the nuclear localization of GFP-mutant p53 protein but not GFP alone in Saos-2 osteosarcoma cells. In addition, ellipticine increased the nuclear localization of endogenous p53 in HCT116 colon cancer cells with a resultant increase in the transactivation of the p21 promoter. Increased nuclear p53 after ellipticine treatment was not associated with an increase in DNA double stranded breaks, indicating that ellipticine shifts p53 to the nucleus through a mechanism independent of DNA damage. Thus, a chemical biology approach has identified a molecule that shifts the localization of p53 and enhances its nuclear activity.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 7%
United States 1 3%
Canada 1 3%
Unknown 26 87%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 27%
Student > Ph. D. Student 6 20%
Student > Bachelor 3 10%
Other 3 10%
Professor 2 7%
Other 7 23%
Unknown 1 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 33%
Chemistry 5 17%
Biochemistry, Genetics and Molecular Biology 4 13%
Medicine and Dentistry 4 13%
Engineering 2 7%
Other 4 13%
Unknown 1 3%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2015.
All research outputs
#3,538,248
of 12,321,253 outputs
Outputs from Apoptosis
#93
of 578 outputs
Outputs of similar age
#78,881
of 266,414 outputs
Outputs of similar age from Apoptosis
#2
of 23 outputs
Altmetric has tracked 12,321,253 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 578 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 51% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,414 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.