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Flow rate dependent continuous hydrolysis of protein isolates

Overview of attention for article published in AMB Express, February 2018
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21 Mendeley
Title
Flow rate dependent continuous hydrolysis of protein isolates
Published in
AMB Express, February 2018
DOI 10.1186/s13568-018-0548-9
Pubmed ID
Authors

Tim Sewczyk, Marieke Hoog Antink, Michael Maas, Stephen Kroll, Sascha Beutel

Abstract

Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 29%
Student > Ph. D. Student 3 14%
Student > Master 3 14%
Student > Doctoral Student 2 10%
Researcher 1 5%
Other 1 5%
Unknown 5 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 19%
Chemical Engineering 3 14%
Agricultural and Biological Sciences 2 10%
Environmental Science 2 10%
Philosophy 1 5%
Other 3 14%
Unknown 6 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2018.
All research outputs
#7,835,537
of 12,487,420 outputs
Outputs from AMB Express
#352
of 778 outputs
Outputs of similar age
#194,148
of 343,045 outputs
Outputs of similar age from AMB Express
#9
of 31 outputs
Altmetric has tracked 12,487,420 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 778 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 343,045 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.