Title |
Electron transfer dissociation of peptide anions
|
---|---|
Published in |
Journal of the American Society for Mass Spectrometry, June 2005
|
DOI | 10.1016/j.jasms.2005.01.015 |
Pubmed ID | |
Authors |
Joshua J. Coon, Jeffrey Shabanowitz, Donald F. Hunt, John E. P. Syka |
Abstract |
Ion/ion reactions of multiply deprotonated peptide anions with xenon radical cations result in electron abstraction to generate charge-reduced peptide anions containing a free-radical site. Peptide backbone cleavage then occurs by hydrogen radical abstraction from a backbone amide N to facilitate cleavage of the adjacent C-C bond, thereby producing a- and x-type product ions. Introduction of free-radical sites to multiply charged peptides allows access to new fragmentation pathways that are otherwise too costly (e.g., lowers activation energies). Further, ion/ion chemistry, namely electron transfer reactions, presents a rapid and efficient means of generating odd-electron multiply charged peptides; these reactions can be used for studying gas-phase chemistries and for peptide sequence analysis. |
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Other | 18 | 14% |
Unknown | 16 | 12% |
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