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Gibberellin biosynthesis in Gibberella fujikuroi: cloning and characterization of the copalyl diphosphate synthase gene

Overview of attention for article published in Current Genetics, September 1998
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Title
Gibberellin biosynthesis in Gibberella fujikuroi: cloning and characterization of the copalyl diphosphate synthase gene
Published in
Current Genetics, September 1998
DOI 10.1007/s002940050392
Pubmed ID
Authors

B. Tudzynski, H. Kawaide, Y. Kamiya

Abstract

The gene coding for copalyl diphosphate synthase (CPS), which represents the first gene of the gibberellin pathway, was isolated from the rice pathogen Gibberella fujikuroi. This fungus is used commercially for the production of gibberellic acid and related gibberellins. CPS is a terpene cyclase which catalyzes the first specific step of the gibberellin (GA) pathway as it branches off from the general isoprenoid (biosynthetic) pathway at geranylgeranyl disphosphate (GGDP). A cDNA fragment of the cps gene from the fungus G. fujikuroi was amplified by RT-PCR using oligonucleotides based on amino-acid sequences which were conserved between the plant CPSs and the bifunctional CPS/KS of the fungus Phaeosphaeria sp. L487. A 588-bp fragment obtained with nested PCR was used to isolate the corresponding genomic clone of the cps gene from the wild-type lambda-library. This gene consists of three exons and two introns. The three exons are 2877 bp long and encode 959 amino-acid residues. The protein shares 48% identity with the bifunctional Phaeosphaeria sp. L487 FCPS and between 16% and 18% identity to the corresponding plant CPSs. Expression of the G. fujikuroi cps gene is strongly enhanced under conditions optimized for gibberellin biosynthesis and is reduced when high amounts of ammonium are present in the medium. Gene disruption, followed by gibberellin assays and Southern-blot analysis of the transformants, demonstrated clearly that the cloned gene has the expected function in the biosynthesis of fungal gibberellins.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 2 5%
Japan 1 2%
United States 1 2%
Germany 1 2%
Unknown 39 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 25%
Student > Ph. D. Student 8 18%
Professor > Associate Professor 6 14%
Student > Master 6 14%
Student > Bachelor 3 7%
Other 6 14%
Unknown 4 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 52%
Chemistry 6 14%
Biochemistry, Genetics and Molecular Biology 6 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Medicine and Dentistry 1 2%
Other 1 2%
Unknown 6 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2020.
All research outputs
#8,535,684
of 25,374,917 outputs
Outputs from Current Genetics
#345
of 1,232 outputs
Outputs of similar age
#10,000
of 31,199 outputs
Outputs of similar age from Current Genetics
#1
of 5 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,232 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 31,199 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them