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Cost-effective downstream processing of recombinantly produced pexiganan peptide and its antimicrobial activity

Overview of attention for article published in AMB Express, January 2018
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Title
Cost-effective downstream processing of recombinantly produced pexiganan peptide and its antimicrobial activity
Published in
AMB Express, January 2018
DOI 10.1186/s13568-018-0541-3
Pubmed ID
Authors

Baode Sun, David Wibowo, Anton P. J. Middelberg, Chun-Xia Zhao

Abstract

Antimicrobial peptides (AMPs) have significant potential as alternatives to classical antibiotics. However, AMPs are currently prepared using processes which are often laborious, expensive and of low-yield, thus hindering their research and application. Large-scale methods for production of AMPs using a cost-effective approach is urgently required. In this study, we report a scalable, chromatography-free downstream processing method for producing an antimicrobial peptide, pexiganan, using recombinant Escherichia coli (E. coli). The four helix bundle structure of the unique carrier protein DAMP4 was used to facilitate a simple and cheap purification process based on a selective thermochemical precipitation. Highly pure fusion protein DAMP4var-pexiganan was obtained at high yield (around 24 mg per 800 mL cell culture with a final cultivation OD600 ~ 2). The purification yield of DAMP4var-pexiganan protein is increased twofold with a 72.9% of the protein recovery in this study as compared to the previous purification processes (Dwyer in Chem Eng Sci 105:12-21, 2014). The antimicrobial peptide pexiganan was released and activated from the fusion protein by a simple acid-cleavage. Isoelectric precipitation was then applied to separate the pexiganan peptide from the DAMP4var protein carrier. The final yield of pure bio-produced pexiganan was 1.6 mg from 800 mL of bacterial cell culture (final cultivation OD600 ~ 2). The minimum bactericidal concentration (MBC) test demonstrated that the bio-produced pexiganan has the same antimicrobial activity as chemically synthesized counterpart. This novel downstream process provides a new strategy for simple and probable economic production of antimicrobial peptides.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 22%
Student > Bachelor 8 18%
Student > Master 5 11%
Researcher 4 9%
Professor 4 9%
Other 4 9%
Unknown 10 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 29%
Chemistry 6 13%
Agricultural and Biological Sciences 4 9%
Immunology and Microbiology 3 7%
Chemical Engineering 1 2%
Other 3 7%
Unknown 15 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2018.
All research outputs
#7,787,157
of 12,413,055 outputs
Outputs from AMB Express
#346
of 766 outputs
Outputs of similar age
#192,338
of 339,799 outputs
Outputs of similar age from AMB Express
#12
of 37 outputs
Altmetric has tracked 12,413,055 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 766 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
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We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.