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Microbial diversity of saline environments: searching for cytotoxic activities

Overview of attention for article published in AMB Express, December 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

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1 blog
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Citations

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50 Mendeley
Title
Microbial diversity of saline environments: searching for cytotoxic activities
Published in
AMB Express, December 2017
DOI 10.1186/s13568-017-0527-6
Pubmed ID
Authors

Carolina Díaz-Cárdenas, Angela Cantillo, Laura Yinneth Rojas, Tito Sandoval, Susana Fiorentino, Jorge Robles, Freddy A. Ramos, María Mercedes Zambrano, Sandra Baena

Abstract

In order to select halophilic microorganisms as a source of compounds with cytotoxic activities, a total of 135 bacterial strains were isolated from water and sediment samples collected from the Zipaquirá salt mine in the Colombian Andes. We determined the cytotoxic effects of 100 crude extracts from 54 selected organisms on the adherent murine mammary cell carcinoma 4T1 and human mammary adenocarcinoma MCF-7 cell lines. These extracts were obtained from strains of Isoptericola, Ornithinimicrobium, Janibacter, Nesterenkonia, Alkalibacterium, Bacillus, Halomonas, Chromohalobacter, Shewanella, Salipiger, Martellela, Oceanibaculum, Caenispirillum and Labrenzia. The extracts of 23 strains showed an IC50 of less than 100 μg mL-1. They were subsequently analyzed by LC/MS allowing dereplication of 20 compounds. The cytotoxic effect was related to a complex mixture of diketopiperazines present in many of the extracts analyzed. The greatest cytotoxic activity against both of the evaluated cell lines was obtained from the chloroform extract of Labrenzia aggregata USBA 371 which had an IC50 < 6 μg mL-1. Other extracts with high levels of cytotoxic activity were obtained from Bacillus sp. (IC50 < 50 μg mL-1) which contained several compounds such as macrolactin L and A, 7-O-succinoylmacrolactin F and iturin. Shewanella chilikensis USBA 344 also showed high levels of cytotoxic activity against both cell lines in the crude extract: an IC50 < 15 μg mL-1 against the 4T1 cell line and an IC50 < 68 μg mL-1 against the MCF-7 cell line. Nesterenkonia sandarakina CG 35, which has an IC50 of 118 µg mL-1 against 4T1, is a producer of diketopiperazines and 1-acetyl-β-carboline. Also, Ornithinimicrobium kibberense CG 24, which has IC50 < 50 μg mL-1, was a producer of diketopiperazines and lagunamycin. Our study demonstrates that these saline environments are habitats of halophilic and halotolerant bacteria that have previously unreported cytotoxic activity.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 16%
Student > Ph. D. Student 6 12%
Student > Master 4 8%
Student > Doctoral Student 4 8%
Student > Bachelor 4 8%
Other 8 16%
Unknown 16 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 20%
Immunology and Microbiology 8 16%
Biochemistry, Genetics and Molecular Biology 7 14%
Chemistry 4 8%
Environmental Science 1 2%
Other 5 10%
Unknown 15 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 June 2020.
All research outputs
#3,689,457
of 23,012,811 outputs
Outputs from AMB Express
#63
of 1,241 outputs
Outputs of similar age
#80,339
of 440,922 outputs
Outputs of similar age from AMB Express
#4
of 45 outputs
Altmetric has tracked 23,012,811 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,241 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 440,922 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.