| Title |
An aptamer-based biosensor for detection of doxorubicin by electrochemical impedance spectroscopy
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, December 2017
|
| DOI | 10.1007/s00216-017-0786-8 |
| Pubmed ID | |
| Authors |
Nicole Bahner, Peggy Reich, Dieter Frense, Marcus Menger, Katharina Schieke, Dieter Beckmann |
| Abstract |
An aptamer-based biosensor was developed for the detection of doxorubicin using electrochemical impedance spectroscopy. Doxorubicin and its 14-dehydroxylated version daunorubicin are anthracyclines often used in cancer treatment. Due to their mutagenic and cardiotoxic effects, detection in groundwater is desirable. We developed a biosensor using the daunorubicin-binding aptamer as biological recognition element. The aptamer was successfully co-immobilized with mercaptohexanol on gold and a density of 1.3*1013 ± 2.4*1012 aptamer molecules per cm2 was achieved. The binding of doxorubicin to the immobilized aptamer was detected by electrochemical impedance spectroscopy. The principle is based on the inhibition of electron transfer between electrode and ferro-/ferricyanide in solution caused by the binding of doxorubicin to the immobilized aptamer. A linear relationship between the charge transfer resistance (R ct ) and the doxorubicin concentration was obtained over the range of 31 nM to 125 nM doxorubicin, with an apparent binding constant of 64 nM and a detection limit of 28 nM. With the advantages of high sensitivity, selectivity, and simple sensor construction, this method shows a high potential of impedimetric aptasensors in environmental monitoring. Graphical abstract Measurement chamber and immobilization principle for the detection of doxorubicin by electrochemical impedance spectroscopy. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Germany | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 50% |
| Members of the public | 1 | 25% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 77 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 16% |
| Researcher | 11 | 14% |
| Student > Master | 9 | 12% |
| Student > Bachelor | 7 | 9% |
| Student > Doctoral Student | 6 | 8% |
| Other | 5 | 6% |
| Unknown | 27 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 17% |
| Chemistry | 12 | 16% |
| Engineering | 7 | 9% |
| Agricultural and Biological Sciences | 6 | 8% |
| Materials Science | 3 | 4% |
| Other | 5 | 6% |
| Unknown | 31 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2018.
All research outputs
#15,745,807
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,753
of 9,619 outputs
Outputs of similar age
#243,697
of 445,640 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#44
of 154 outputs
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