Title |
Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing
|
---|---|
Published in |
AMB Express, December 2014
|
DOI | 10.1186/s13568-014-0086-z |
Pubmed ID | |
Authors |
Clara Navarrete, Jens Nielsen, Verena Siewers |
Abstract |
Ethanol is by volume the largest fermentation product. During ethanol production by Saccharomyces cerevisiae about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed for improving the ethanol yield while reducing glycerol production. Here we studied the effect of reducing glycerol export/formation through deletion of the aquaglyceroporin gene FPS1 together with expressing gapN encoding NADP(+)-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans and overexpressing the ATP-NADH kinase gene UTR1 from S. cerevisiae. This strategy will allow reducing the redox balance problem observed when the glycerol pathway is blocked, and hereby improve ethanol production. We found that our strategy enabled increasing the ethanol yield by 4.6% in the case of the best producing strain, compared to the reference strain, without any major effect on the specific growth rate. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Thailand | 1 | 1% |
Unknown | 71 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 14 | 19% |
Student > Bachelor | 10 | 14% |
Student > Ph. D. Student | 9 | 13% |
Student > Doctoral Student | 5 | 7% |
Student > Master | 5 | 7% |
Other | 11 | 15% |
Unknown | 18 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 23 | 32% |
Biochemistry, Genetics and Molecular Biology | 17 | 24% |
Engineering | 5 | 7% |
Chemical Engineering | 4 | 6% |
Computer Science | 1 | 1% |
Other | 3 | 4% |
Unknown | 19 | 26% |