Title |
Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment
|
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Published in |
Journal of Neuro-Oncology, September 2012
|
DOI | 10.1007/s11060-012-0973-6 |
Pubmed ID | |
Authors |
Rintaro Hashizume, Ivan Smirnov, Sharon Liu, Joanna J. Phillips, Jeanette Hyer, Tracy R. McKnight, Michael Wendland, Michael Prados, Anu Banerjee, Theodore Nicolaides, Sabine Mueller, Charles D. James, Nalin Gupta |
Abstract |
Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 1% |
United States | 1 | 1% |
Unknown | 79 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 17 | 21% |
Student > Ph. D. Student | 12 | 15% |
Student > Master | 8 | 10% |
Student > Bachelor | 6 | 7% |
Other | 6 | 7% |
Other | 12 | 15% |
Unknown | 20 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 16 | 20% |
Agricultural and Biological Sciences | 15 | 19% |
Biochemistry, Genetics and Molecular Biology | 11 | 14% |
Neuroscience | 8 | 10% |
Engineering | 3 | 4% |
Other | 6 | 7% |
Unknown | 22 | 27% |