Title |
Assessment of circulating Wnt1 inducible signalling pathway protein 1 (WISP‐1)/CCN4 as a novel biomarker of obesity
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Published in |
Journal of Cell Communication and Signaling, November 2017
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DOI | 10.1007/s12079-017-0427-1 |
Pubmed ID | |
Authors |
Christopher Tacke, Krasimira Aleksandrova, Miriam Rehfeldt, Veronica Murahovschi, Mariya Markova, Margrit Kemper, Silke Hornemann, Ulrike Kaiser, Caroline Honig, Christiana Gerbracht, Stefan Kabisch, Tina Hörbelt, D. Margriet Ouwens, Martin O. Weickert, Heiner Boeing, Andreas F. H. Pfeiffer, Olga Pivovarova, Natalia Rudovich |
Abstract |
WNT1 inducible signaling pathway protein 1 (WISP-1/CCN4) is a novel adipokine, which is upregulated in obesity, and induces a pro-inflammatory response in macrophages in-vitro. Preclinical observations suggested WISP-1/CCN4 as a potential candidate for novel obesity therapy targeting adipose tissue inflammation. Whether circulating levels of WISP-1/CCN4 in humans are altered in obesity and/or type 2 diabetes (T2DM) and in the postprandial state, however, is unknown. This study assessed circulating WISP-1/CCN4 levels in a) paired liquid meal tests and hyperinsulinemic- euglycemic clamps (cohort I, n = 26), b) healthy individuals (cohort II, n = 207) and c) individuals with different stages of obesity and glucose tolerance (cohort III, n = 253). Circulating plasma and serum WISP-1/CCN4 concentrations were measured using a commercially available ELISA. WISP-1/CCN4 levels were not influenced by changes in insulin and/or glucose during the tests. In healthy individuals, WISP-1/CCN4 was detectable in 13% of plasma samples with the intraclass correlation coefficient of 0.93 (95% CI: 0.84-0.96) and in 58.1% of the serum samples in cohort III. Circulating WISP-1/CCN4 positively correlated with body mass index, body fat percentage, leptin and triglyceride levels, hip circumference and fatty liver index. No differences in WISP-1/CCN4 levels between individuals with normal glucose tolerance, impaired glucose tolerance and T2DM were found. The circulating concentrations of WISP-1/CCN4 showed no acute regulation in postprandial state and correlated with anthropometrical obesity markers and lipid profiles. In healthy individuals, WISP-1/CCN4 levels are more often below the detection limit. Thus, serum WISP-1/CCN4 levels may be used as a suitable biomarker of obesity. |
X Demographics
Geographical breakdown
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Spain | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 18% |
Other | 3 | 9% |
Student > Bachelor | 3 | 9% |
Student > Master | 3 | 9% |
Student > Ph. D. Student | 3 | 9% |
Other | 6 | 18% |
Unknown | 9 | 27% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 12 | 36% |
Biochemistry, Genetics and Molecular Biology | 4 | 12% |
Agricultural and Biological Sciences | 2 | 6% |
Unspecified | 1 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 12% |
Unknown | 9 | 27% |