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Investigations to extend viability of a rainbow trout primary gill cell culture

Overview of attention for article published in Ecotoxicology, November 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (52nd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

Mentioned by

twitter
3 tweeters

Citations

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4 Dimensions

Readers on

mendeley
11 Mendeley
Title
Investigations to extend viability of a rainbow trout primary gill cell culture
Published in
Ecotoxicology, November 2017
DOI 10.1007/s10646-017-1856-6
Pubmed ID
Authors

Richard J. Maunder, Matthew G. Baron, Stewart F. Owen, Awadhesh N. Jha

Abstract

The primary culture of fish gill cells can provide functional, cell diverse, model in vitro platforms able to tolerate an aqueous exposure analogous to in vivo tissues. The utility of such models could be extended to a variety of longer term exposure scenarios if a method could be established to extend culture viability when exposed to water for longer periods. Here we report findings of a series of experiments to establish increased longevity, as monitored by culture transepithelial electrical resistance (TEER) and concurrent histological developments. Experimental cultures improved TEER during apical freshwater exposure for a mean of twelve days, compared to previous viabilities of up to 3 days. Cultures with larger surface areas and the use of trout serum rather than foetal bovine serum (FBS) contributed to the improvement, while perfusion of the intact gill prior to cell harvest resulted in a significantly faster preparation. Detailed scanning electron microscopy analysis of cultures revealed diverse surface structures that changed with culture age. Cultures grown on membranes with an increased porosity, collagen coating or 3D structure were of no benefit compared to standard membranes. Increased culture longevity, achieved in this study and reported for the first time, is a significant breakthrough and opens up a variety of future experimentation that has previously not been possible. The extended viability facilitates exploration of in vitro chronic or pulse-exposure test paradigms, longer term physiological and environmental monitoring studies and the potential for interactive co-culture with other organoid micro-tissues.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 27%
Student > Doctoral Student 3 27%
Student > Ph. D. Student 2 18%
Student > Bachelor 1 9%
Student > Master 1 9%
Other 1 9%
Readers by discipline Count As %
Environmental Science 3 27%
Biochemistry, Genetics and Molecular Biology 2 18%
Agricultural and Biological Sciences 2 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Earth and Planetary Sciences 1 9%
Other 0 0%
Unknown 2 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 November 2017.
All research outputs
#6,927,497
of 12,124,842 outputs
Outputs from Ecotoxicology
#275
of 901 outputs
Outputs of similar age
#133,089
of 281,864 outputs
Outputs of similar age from Ecotoxicology
#10
of 28 outputs
Altmetric has tracked 12,124,842 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 901 research outputs from this source. They receive a mean Attention Score of 2.7. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,864 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.