| Title |
Characterization of a beta-glucosidase from Bacillus licheniformis and its effect on bioflocculant degradation
|
|---|---|
| Published in |
AMB Express, November 2017
|
| DOI | 10.1186/s13568-017-0501-3 |
| Pubmed ID | |
| Authors |
Zhen Chen, Tong Meng, Zhipeng Li, Peize Liu, Yuanpeng Wang, Ning He, Dafeng Liang |
| Abstract |
Bacillus licheniformis CGMCC 2876, an aerobic spore-forming bacterium, produces a polysaccharide bioflocculant that is biodegradable and harmless. The present study determined that β-glucosidase played a negative role in bioflocculant synthesis. The gene encoding β-glucosidase was cloned and expressed in Escherichia coli BL21. This gene consists of 1437 bp and encodes 478 amino acid residues. The recombinant β-glucosidase (Bgl.bli1) was purified and showed a molecular mass of 53.4 kDa by SDS-PAGE. The expression and reaction conditions of Bgl.bli1 were optimized; the activity of β-glucosidase reached a maximum at 45.44 U/mL. Glucose clearly inhibited the activity of β-glucosidase. The purified recombinant Bgl.bli1 hydrolysed polysaccharide bioflocculant in vitro and synergised with other cellulases. The ability of Bgl.bli1 to hydrolyse polysaccharide bioflocculant was the reason for the decrease in flocculating activity and indicated the utility of this enzyme for diverse industrial processes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 17% |
| Student > Master | 5 | 14% |
| Lecturer | 3 | 8% |
| Student > Bachelor | 3 | 8% |
| Other | 3 | 8% |
| Other | 5 | 14% |
| Unknown | 11 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 22% |
| Biochemistry, Genetics and Molecular Biology | 5 | 14% |
| Immunology and Microbiology | 3 | 8% |
| Chemical Engineering | 2 | 6% |
| Environmental Science | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 14 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2017.
All research outputs
#15,482,347
of 23,007,053 outputs
Outputs from AMB Express
#447
of 1,240 outputs
Outputs of similar age
#207,169
of 330,777 outputs
Outputs of similar age from AMB Express
#20
of 41 outputs
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