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NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level

Overview of attention for article published in AMB Express, October 2014
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Mentioned by

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2 tweeters

Citations

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26 Dimensions

Readers on

mendeley
75 Mendeley
Title
NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level
Published in
AMB Express, October 2014
DOI 10.1186/s13568-014-0081-4
Pubmed ID
Authors

Jan Dines Knudsen, Magnus Carlquist, Marie Gorwa-Grauslund

Abstract

A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase double deletion strain (gpd1Δgpd2Δ), which is deficient in the ability to regenerate NAD(+) via glycerol formation. The responsiveness of the reporter system to externally induced perturbations in NADH oxidation was also evaluated in the gpd1Δgpd2Δ strain background by addition of acetoin, as well as by introduction of a set of heterologous xylose reductases (XRs) having different selectivities for NADH. Addition of acetoin during cell proliferation under oxygen-limited conditions resulted in a more than 2-fold decrease in mean fluorescence intensity as compared to the control experiment. Strains carrying XRs with different selectivities for NADH could be distinguished at the single cell level, so that the XR with the highest selectivity for NADH displayed the lowest fluorescence. In conclusion, the designed system successfully allowed for monitoring perturbations in the cellular redox metabolism caused by environmental changes, or by heterologous gene expression. The reporter system displayed high resolution in distinguishing cytosolic NADH oxidation capacity and hence has potential to be used for high-throughput screening based on the fluorescence of single cells.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
United States 1 1%
Brazil 1 1%
Unknown 72 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 21%
Researcher 16 21%
Student > Master 8 11%
Student > Bachelor 8 11%
Other 6 8%
Other 13 17%
Unknown 8 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 40%
Biochemistry, Genetics and Molecular Biology 19 25%
Chemical Engineering 4 5%
Engineering 4 5%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Other 4 5%
Unknown 13 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2014.
All research outputs
#17,219,905
of 21,321,610 outputs
Outputs from AMB Express
#761
of 1,176 outputs
Outputs of similar age
#179,791
of 252,521 outputs
Outputs of similar age from AMB Express
#33
of 66 outputs
Altmetric has tracked 21,321,610 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,176 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,521 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 66 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.