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Improved thermostability of a metagenomic glucose-tolerant β-glycosidase based on its X-ray crystal structure

Overview of attention for article published in Applied Microbiology & Biotechnology, October 2017
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Title
Improved thermostability of a metagenomic glucose-tolerant β-glycosidase based on its X-ray crystal structure
Published in
Applied Microbiology & Biotechnology, October 2017
DOI 10.1007/s00253-017-8525-9
Pubmed ID
Authors

Tomohiko Matsuzawa, Masahiro Watanabe, Katsuro Yaoi

Abstract

MeBglD2, a metagenomic β-glycosidase, is stimulated by various saccharides, including D-glucose, D-xylose, and maltose, and it promotes the enzymatic saccharification of plant biomass. To improve the thermostability of MeBglD2, its X-ray crystal structure was analyzed, and the amino acid residues responsible for its thermostability were identified using the structural information. Mutations in His8, Asn59, and Gly295 improved the thermostability of MeBglD2, and the combination of these mutations resulted in the highest thermostability. Compared with wild-type MeBglD2, thermostable MeBglD2 mutants promoted plant biomass saccharification using Trichoderma reesei cellulase. In addition to thermostability, the thermostable mutants exhibited higher tolerance to ethanol, dimethyl sulfoxide, and copper ions, indicating that the MeBglD2 mutants generated in this study were improved in their tolerance to not only high temperature but also to organic solvents and metal ions.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Student > Ph. D. Student 2 25%
Student > Master 2 25%
Student > Bachelor 1 13%
Unspecified 1 13%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 50%
Unspecified 2 25%
Biochemistry, Genetics and Molecular Biology 1 13%
Chemistry 1 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2017.
All research outputs
#10,686,744
of 12,050,803 outputs
Outputs from Applied Microbiology & Biotechnology
#4,502
of 5,188 outputs
Outputs of similar age
#238,852
of 284,726 outputs
Outputs of similar age from Applied Microbiology & Biotechnology
#83
of 106 outputs
Altmetric has tracked 12,050,803 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,188 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 284,726 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 106 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.