↓ Skip to main content

Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells

Overview of attention for article published in Annals of Biomedical Engineering, September 2014
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
19 Dimensions

Readers on

mendeley
21 Mendeley
Title
Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells
Published in
Annals of Biomedical Engineering, September 2014
DOI 10.1007/s10439-014-1114-5
Pubmed ID
Authors

Yang Gao, Jennifer Petsche Connell, Lalita Wadhwa, Rodrigo Ruano, Jeffrey G. Jacot

Abstract

Amniotic fluid-derived stem cells (AFSC) have been shown to be broadly multipotent and non-tumorogenic. Previous studies of direct mixing of AFSC and neonatal rat ventricle myocytes indicated evidence of AFSC cardiogenesis. In this study, we examined human AFSC cardiogenic potential in indirect co-culture with human cardiac cells in conditions that eliminated the possibility of cell fusion. Human AFSC in contact with human cardiac cells showed expression of cardiac troponin T (cTnT) in immunohistochemistry, and no evidence of cell fusion was found through fluorescent in situ hybridization. When indirectly co-cultured with cardiac cells, human AFSC in contact with cardiac cells across a thin porous membrane showed a statistically significant increase in cTnT expression compared to non-contact conditions but lacked upregulation of calcium modulating proteins and did not have functional or morphological characteristics of mature cardiomyocytes. This suggests that contact is a necessary but not sufficient condition for AFSC cardiac differentiation in co-culture with cardiac cells.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 33%
Researcher 5 24%
Student > Master 4 19%
Professor 1 5%
Lecturer 1 5%
Other 1 5%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 29%
Medicine and Dentistry 5 24%
Biochemistry, Genetics and Molecular Biology 4 19%
Engineering 2 10%
Materials Science 1 5%
Other 0 0%
Unknown 3 14%