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Novel archaeal thermostable cellulases from an oil reservoir metagenome

Overview of attention for article published in AMB Express, September 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#18 of 993)
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

blogs
1 blog
twitter
9 tweeters
googleplus
1 Google+ user

Citations

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17 Dimensions

Readers on

mendeley
49 Mendeley
Title
Novel archaeal thermostable cellulases from an oil reservoir metagenome
Published in
AMB Express, September 2017
DOI 10.1186/s13568-017-0485-z
Pubmed ID
Authors

Anna Lewin, Jinglie Zhou, Vu Thuy Trang Pham, Tone Haugen, Mohamed El Zeiny, Olav Aarstad, Wolfgang Liebl, Alexander Wentzel, Mark R. Liles

Abstract

Microbial assemblages were sampled from an offshore deep sub-surface petroleum reservoir 2.5 km below the ocean floor off the coast of Norway, providing conditions of high temperature and pressure, to identify new thermostable enzymes. In this study, we used DNA sequences obtained directly from the sample metagenome and from a derived fosmid library to survey the functional diversity of this extreme habitat. The metagenomic fosmid library containing 11,520 clones was screened using function- and sequence-based methods to identify recombinant clones expressing carbohydrate-degrading enzymes. Open reading frames (ORFs) encoding carbohydrate-degrading enzymes were predicted by BLAST against the CAZy database, and many fosmid clones expressing carbohydrate-degrading activities were discovered by functional screening using Escherichia coli as a heterologous host. Each complete ORF predicted to encode a cellulase identified from sequence- or function-based screening was subcloned in an expression vector. Five subclones was found to have significant activity using a fluorescent cellulose model substrate, and three of these were observed to be highly thermostable. Based on phylogenetic analyses, the thermostable cellulases were derived from thermophilic Archaea and are distinct from known cellulases. Cellulase F1, obtained from function-based screening, contains two distinct cellulase modules, perhaps resulting from fusion of two archaeal cellulases and with a novel protein structure that may result in enhanced activity and thermostability. This enzyme was found to exhibit exocellulase function and to have a remarkably high activity compared to commercially available enzymes. Results from this study highlight the complementarity of hybrid approaches for enzyme discovery, combining sequence- and function-based screening.

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 49 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 22%
Student > Bachelor 9 18%
Student > Master 8 16%
Researcher 6 12%
Student > Doctoral Student 2 4%
Other 2 4%
Unknown 11 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 31%
Agricultural and Biological Sciences 10 20%
Immunology and Microbiology 4 8%
Environmental Science 3 6%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Other 4 8%
Unknown 11 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 October 2017.
All research outputs
#1,825,421
of 15,825,604 outputs
Outputs from AMB Express
#18
of 993 outputs
Outputs of similar age
#46,773
of 279,169 outputs
Outputs of similar age from AMB Express
#1
of 29 outputs
Altmetric has tracked 15,825,604 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 993 research outputs from this source. They receive a mean Attention Score of 2.2. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,169 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.