↓ Skip to main content

Correction of β-thalassemia mutant by base editor in human embryos

Overview of attention for article published in Protein & Cell, September 2017
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#4 of 820)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

news
42 news outlets
blogs
9 blogs
twitter
186 X users
patent
24 patents
facebook
7 Facebook pages
googleplus
3 Google+ users
video
1 YouTube creator

Citations

dimensions_citation
187 Dimensions

Readers on

mendeley
408 Mendeley
citeulike
2 CiteULike
Title
Correction of β-thalassemia mutant by base editor in human embryos
Published in
Protein & Cell, September 2017
DOI 10.1007/s13238-017-0475-6
Pubmed ID
Authors

Puping Liang, Chenhui Ding, Hongwei Sun, Xiaowei Xie, Yanwen Xu, Xiya Zhang, Ying Sun, Yuanyan Xiong, Wenbin Ma, Yongxiang Liu, Yali Wang, Jianpei Fang, Dan Liu, Zhou Songyang, Canquan Zhou, Junjiu Huang

Abstract

β-Thalassemia is a global health issue, caused by mutations in the HBB gene. Among these mutations, HBB -28 (A>G) mutations is one of the three most common mutations in China and Southeast Asia patients with β-thalassemia. Correcting this mutation in human embryos may prevent the disease being passed onto future generations and cure anemia. Here we report the first study using base editor (BE) system to correct disease mutant in human embryos. Firstly, we produced a 293T cell line with an exogenous HBB -28 (A>G) mutant fragment for gRNAs and targeting efficiency evaluation. Then we collected primary skin fibroblast cells from a β-thalassemia patient with HBB -28 (A>G) homozygous mutation. Data showed that base editor could precisely correct HBB -28 (A>G) mutation in the patient's primary cells. To model homozygous mutation disease embryos, we constructed nuclear transfer embryos by fusing the lymphocyte or skin fibroblast cells with enucleated in vitro matured (IVM) oocytes. Notably, the gene correction efficiency was over 23.0% in these embryos by base editor. Although these embryos were still mosaic, the percentage of repaired blastomeres was over 20.0%. In addition, we found that base editor variants, with narrowed deamination window, could promote G-to-A conversion at HBB -28 site precisely in human embryos. Collectively, this study demonstrated the feasibility of curing genetic disease in human somatic cells and embryos by base editor system.

X Demographics

X Demographics

The data shown below were collected from the profiles of 186 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 408 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 408 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 126 31%
Student > Ph. D. Student 53 13%
Researcher 49 12%
Student > Master 43 11%
Other 18 4%
Other 40 10%
Unknown 79 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 168 41%
Agricultural and Biological Sciences 59 14%
Medicine and Dentistry 32 8%
Chemistry 15 4%
Neuroscience 10 2%
Other 36 9%
Unknown 88 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 516. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2024.
All research outputs
#49,616
of 25,593,129 outputs
Outputs from Protein & Cell
#4
of 820 outputs
Outputs of similar age
#976
of 328,055 outputs
Outputs of similar age from Protein & Cell
#2
of 20 outputs
Altmetric has tracked 25,593,129 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 820 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.8. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,055 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.