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Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris

Overview of attention for article published in AMB Express, August 2014
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Title
Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris
Published in
AMB Express, August 2014
DOI 10.1186/s13568-014-0068-1
Pubmed ID
Authors

Narin Kirikyali, Jonathan Wood, Ian F Connerton

Abstract

β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in Pichia pastoris under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. The recombinant enzyme was optimally active at 55°C and pH 4.5 with Km and Vmax values of 1.0 mM and 250 μmol min(-1) mg(-1) respectively against 4-nitrophenyl β-xylopyranoside. Xylose was a competitive inhibitor with a Ki of 2.72 mM, whereas fructose was an uncompetitive inhibitor reducing substrate binding affinity (Km) and conversion efficiency (Vmax). The enzyme was characterised to be an exo-cutting enzyme releasing xylose from the non-reducing ends of β-1,4 linked xylooligosaccharides (X2, X3 and X4). Catalytic conversion of X2, X3 and X4 decreased (Vmax and kcat) with increasing chain length.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 19%
Student > Bachelor 7 17%
Researcher 6 14%
Student > Doctoral Student 4 10%
Student > Master 4 10%
Other 7 17%
Unknown 6 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 40%
Agricultural and Biological Sciences 11 26%
Engineering 2 5%
Social Sciences 1 2%
Immunology and Microbiology 1 2%
Other 2 5%
Unknown 8 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2014.
All research outputs
#20,235,415
of 22,761,738 outputs
Outputs from AMB Express
#965
of 1,231 outputs
Outputs of similar age
#198,501
of 236,954 outputs
Outputs of similar age from AMB Express
#12
of 20 outputs
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