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Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples

Overview of attention for article published in AMB Express, September 2017
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  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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Title
Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
Published in
AMB Express, September 2017
DOI 10.1186/s13568-017-0462-6
Pubmed ID
Authors

Nirmal Chandra Barman, Fatema Tuj Zohora, Keshob Chandra Das, Md. Golam Mowla, Nilufa Akhter Banu, Md. Salimullah, Abu Hashem

Abstract

The study was conducted to select the best promising keratinolytic bacterial strain. A good keratinase positive bacterium isolated from the soil samples of Hazaribagh tannery industrial zone, Dhaka was identified as Arthrobacter genus depending on the conventional techniques and confirmed as Arthrobacter sp. by sequencing 16S rRNA gene. The medium components and culture conditions were optimized to enhance keratinase production through shake flask culture. Keratin and feather powder (10 g/l or 1%) were good substrates for the highest keratinase production along with yeast extract (0.2 g/l or 0.02%) as an organic nitrogen source and potassium nitrate (1 g or 0.1%) as an inorganic nitrogen source. Maximum yield of keratinase was found after 24 h of incubation at 37 °C with an initial pH of 7.0 and inoculums volume 5% under 150 rpm when keratin, yeast extract and potassium nitrate were used as nutrient sources. Keratinase production was more than 5.0-fold increased when all optimized parameters were applied simultaneously. The optimum reaction temperature and pH were determined to be 40 °C and 8.0 respectively for crude keratinase activity. Therefore, Arthrobacter sp. NFH5 might be used for large scale production of keratinase for industrial purposes in less time.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 108 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 19%
Student > Ph. D. Student 11 10%
Researcher 10 9%
Student > Master 10 9%
Student > Doctoral Student 4 4%
Other 12 11%
Unknown 40 37%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 26 24%
Agricultural and Biological Sciences 13 12%
Immunology and Microbiology 9 8%
Chemistry 5 5%
Environmental Science 3 3%
Other 7 6%
Unknown 45 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 September 2017.
All research outputs
#14,955,443
of 23,003,906 outputs
Outputs from AMB Express
#349
of 1,240 outputs
Outputs of similar age
#188,577
of 318,503 outputs
Outputs of similar age from AMB Express
#10
of 37 outputs
Altmetric has tracked 23,003,906 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,240 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 318,503 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.