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Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies

Overview of attention for article published in AMB Express, September 2017
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1 tweeter

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13 Mendeley
Title
Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies
Published in
AMB Express, September 2017
DOI 10.1186/s13568-017-0473-3
Pubmed ID
Authors

Meng Ge, Run-Cheng Li, Tailong Qu, Wenjie Gong, Xing-Long Yu, Changchun Tu

Abstract

A fusion protein SBP-Cap∆41, consisting of Cap∆41 (without 41 amino acids at the N-terminus) protein of porcine circovirus 2 (PCV2) and a streptavidin binding peptide (SBP), was constructed. This fusion protein binds to HRP-labeled streptavidin (HRP-SA) through high affinity between SBP and SA, forming an HRP-streptavidin bound antigen (Hsb-Ag) with both immunoreactivity and enzymatic activity, which can be used in a double-antigen sandwich ELISA for detection of PCV2 antibodies. Comparison of the characteristics of the HSb-Cap∆41 and chemical conjugates of the recombinant Cap∆41 protein showed that the HSb-Cap∆41 based double-antigen sandwich ELISA (HBDS-ELISA) had higher specificity and sensitivity. Use of the HBDS-ELISA detected PCV2-IgG in 9 injected pigs as early as 10 days p.i., 3 days earlier than both a double-antigen sandwich ELISA (DS-ELISA) based on a chemically conjugated antigen, and a commercial indirect ELISA kit.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 23%
Unspecified 2 15%
Researcher 2 15%
Student > Ph. D. Student 1 8%
Student > Master 1 8%
Other 1 8%
Unknown 3 23%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 4 31%
Unspecified 2 15%
Biochemistry, Genetics and Molecular Biology 2 15%
Agricultural and Biological Sciences 2 15%
Unknown 3 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2017.
All research outputs
#7,349,022
of 11,794,580 outputs
Outputs from AMB Express
#307
of 705 outputs
Outputs of similar age
#152,626
of 267,590 outputs
Outputs of similar age from AMB Express
#17
of 37 outputs
Altmetric has tracked 11,794,580 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 705 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 6th percentile – i.e., 6% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,590 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.