Title |
Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies
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Published in |
AMB Express, September 2017
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DOI | 10.1186/s13568-017-0473-3 |
Pubmed ID | |
Authors |
Meng Ge, Run-Cheng Li, Tailong Qu, Wenjie Gong, Xing-Long Yu, Changchun Tu |
Abstract |
A fusion protein SBP-Cap∆41, consisting of Cap∆41 (without 41 amino acids at the N-terminus) protein of porcine circovirus 2 (PCV2) and a streptavidin binding peptide (SBP), was constructed. This fusion protein binds to HRP-labeled streptavidin (HRP-SA) through high affinity between SBP and SA, forming an HRP-streptavidin bound antigen (Hsb-Ag) with both immunoreactivity and enzymatic activity, which can be used in a double-antigen sandwich ELISA for detection of PCV2 antibodies. Comparison of the characteristics of the HSb-Cap∆41 and chemical conjugates of the recombinant Cap∆41 protein showed that the HSb-Cap∆41 based double-antigen sandwich ELISA (HBDS-ELISA) had higher specificity and sensitivity. Use of the HBDS-ELISA detected PCV2-IgG in 9 injected pigs as early as 10 days p.i., 3 days earlier than both a double-antigen sandwich ELISA (DS-ELISA) based on a chemically conjugated antigen, and a commercial indirect ELISA kit. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 16 | 100% |
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Student > Bachelor | 5 | 31% |
Researcher | 2 | 13% |
Student > Ph. D. Student | 1 | 6% |
Unspecified | 1 | 6% |
Student > Postgraduate | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 38% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 4 | 25% |
Biochemistry, Genetics and Molecular Biology | 3 | 19% |
Agricultural and Biological Sciences | 2 | 13% |
Unspecified | 1 | 6% |
Unknown | 6 | 38% |