| Title |
Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii
|
|---|---|
| Published in |
AMB Express, August 2014
|
| DOI | 10.1186/s13568-014-0057-4 |
| Pubmed ID | |
| Authors |
Alma Lorena Almaraz-Delgado, José Flores-Uribe, Víctor Hugo Pérez-España, Edgar Salgado-Manjarrez, Jesús Agustín Badillo-Corona |
| Abstract |
Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have been successfully expressed in the chloroplast using endogenous and chimeric promoter sequences. In some cases, proteins have accumulated to high level, demonstrating that this technology could compete with current production platforms. This review focuses on the works that have engineered the chloroplast of C. reinhardtii with the aim of producing recombinant proteins intended for therapeutical use in humans or animals. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 160 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Mexico | 1 | <1% |
| Israel | 1 | <1% |
| Denmark | 1 | <1% |
| Unknown | 156 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 36 | 23% |
| Student > Ph. D. Student | 32 | 20% |
| Student > Bachelor | 21 | 13% |
| Researcher | 20 | 13% |
| Student > Doctoral Student | 8 | 5% |
| Other | 17 | 11% |
| Unknown | 26 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 69 | 43% |
| Biochemistry, Genetics and Molecular Biology | 39 | 24% |
| Engineering | 5 | 3% |
| Chemistry | 4 | 3% |
| Unspecified | 2 | 1% |
| Other | 11 | 7% |
| Unknown | 30 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2014.
All research outputs
#13,336,247
of 22,760,687 outputs
Outputs from AMB Express
#256
of 1,231 outputs
Outputs of similar age
#109,071
of 231,132 outputs
Outputs of similar age from AMB Express
#5
of 18 outputs
Altmetric has tracked 22,760,687 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,231 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,132 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.